Ohashi Y, Nakai Y, Ikeoka H, Koshimo H, Esaki Y, Kato S
Department of Otolaryngology, Osaka City University Medical School, Japan.
Arch Otorhinolaryngol. 1987;244(2):88-90. doi: 10.1007/BF00458553.
We have used a tissue culture technique and a photoelectric method to examine the direct effect of lipopolysaccharide (LPS) on the ciliary activity present in the eustachian tube. Since LPS possesses the major part of the biological activity of endotoxin, our results show clearly that LPS deteriorates the ciliary activity in a dose-response fashion: LPS does not deteriorate the ciliary activity up to 168 h if its concentration is 1 ng/ml or less; 10 ng/ml LPS can cause deterioration of the ciliary activity with extended exposure (more than 96 h); LPS can cause dysfunction of the cilia rather quickly if the concentration is 100 ng/ml or more. Our results show that the ciliary activity in the eustachian tube under clinical conditions can be affected by endotoxin.
我们采用组织培养技术和光电方法，研究脂多糖（LPS）对咽鼓管中纤毛活动的直接影响。由于LPS具有内毒素的大部分生物活性，我们的结果清楚地表明，LPS以剂量反应方式使纤毛活动恶化：如果LPS浓度为1 ng/ml或更低，在长达168小时内不会使纤毛活动恶化；10 ng/ml的LPS在长时间暴露（超过96小时）时可导致纤毛活动恶化；如果浓度为100 ng/ml或更高，LPS可相当迅速地导致纤毛功能障碍。我们的结果表明，临床条件下咽鼓管中的纤毛活动会受到内毒素的影响。
