Rajagopal Srividyadevi, Chander Naveen Gopi, Anitha Kuttae Viswanathan, Balasubramaniam Muthukumar
Department of Prosthodontics, SRM Dental College, Chennai, Tamil Nadu, India.
Contemp Clin Dent. 2020 Apr-Jun;11(2):116-120. doi: 10.4103/ccd.ccd_63_20. Epub 2020 Aug 7.
Clinical studies have established mastication as a stress relaxation behavior in humans. Absence of teeth compromises mastication, increasing psychologic stress in individuals depicted by many physiologic changes in body. Quantitative level of psychologic stress bio-markers serve as indicators of underlying physical ailment. Lesser literatures are available in determining the role of alpha amylase stress bio marker in partially edentulous clinical situations.
The purpose of this clinical study was to evaluate the levels of salivary alpha-amylase (sAA) stress biomarker in partially edentulous subjects before and after restoration with fixed dental prosthesis.
Forty partially edentulous patients with missing mandibular first molar were selected for this study. Two questionnaires, state trait anxiety inventory (STAI) and perceived stress scale (PSS) was used to evaluate stress and anxiety levels of participants. The recruited participants were treated with metal ceramic fixed dental prosthesis (FDP). A visual analog scale (VAS) was used to determine the patient satisfaction .Unstimulated salivary samples were collected preoperative, 3rd and 6th month post FDP placement. Level of sAA was estimated. Data obtained in the form of mean ± SD was subjected to statistical analysis using paired sample -test (α=.05).
The salivary alpha amylase level was highest with mean of 36.73 μM/min/mg ptn before restoration with FDP. In the third month after prosthesis placement, the enzyme values decreased to16.62 μM/min/mg ptn and least value of 8.58 μM/min/mg ptn was detected in sixth month ( < 0.05).
The salivary alpha amylase stress biomarker decreased after tooth replacement with FDP.
临床研究已证实咀嚼是人类的一种应激放松行为。牙齿缺失会影响咀嚼，导致身体出现许多生理变化，进而增加个体的心理压力。心理应激生物标志物的定量水平可作为潜在身体疾病的指标。关于α淀粉酶应激生物标志物在部分牙列缺损临床情况中的作用，相关文献较少。
本临床研究旨在评估部分牙列缺损患者在固定义齿修复前后唾液α淀粉酶（sAA）应激生物标志物的水平。
本研究选取了40例下颌第一磨牙缺失的部分牙列缺损患者。使用两份问卷，即状态特质焦虑量表（STAI）和感知压力量表（PSS）来评估参与者的压力和焦虑水平。招募的参与者接受金属陶瓷固定义齿（FDP）治疗。使用视觉模拟量表（VAS）确定患者满意度。在术前、FDP放置后第3个月和第6个月收集非刺激性唾液样本。评估sAA水平。以均值±标准差形式获得的数据采用配对样本t检验进行统计分析（α = 0.05）。
在FDP修复前，唾液α淀粉酶水平最高，平均为36.73μM/min/mg蛋白。在假体放置后的第三个月，酶值降至16.62μM/min/mg蛋白，在第六个月检测到最低值8.58μM/min/mg蛋白（P < 0.05）。
用FDP修复牙齿后，唾液α淀粉酶应激生物标志物降低。
