Rashkova Maya R, Ribagin Lora S, Toneva Nina G
Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University, Sofia, Bulgaria.
Folia Med (Plovdiv). 2012 Apr-Jun;54(2):46-51. doi: 10.2478/v10153-011-0088-4.
Salivary alpha-amylase is a useful biomarker that can be used in assessing human psychobiological and social behavioural processes. Studying it opens up possibilities for the creation of novel concepts concerning the interaction of biological and social processes and their impact on health and behaviour.
The levels of salivary alpha-amylase and situation anxiety self-assessment using Spielberger test were measured twice in 30 individuals aged 21.37 +/- 0.96 yrs (18 females and 12 males): once during stressful situation (prior to examination) and, again a month later, in stress-free environment (during a training session). Salivary alpha-amylase was measured using kinetic reaction kit Salimetrics LLC--USA.
The mean level of salivary alpha-amylase measured during the first measurement 156.0 +/- 93.33 U/ml. During the second measurement in the absence of intense stress, the levels were two times lower - 74.03 +/- 58.06 U/ml and the differences were statistically significant (P < 0.001). We found a statistically significant correlation between the levels of salivary alpha-amylase in both measurements (P < 0.01). The correlation coefficient was r = 0.472 (P < 0.01).
The adapted version of the State-Trait Anxiety Inventory score (STAI) created by Spielberger is appropriate for assessment of stress-related anxiety in young individuals. Salivary alpha-amylase may be used as a biomarker for objective evaluation of the psychosomatic state of individuals in a stressful environment. The combination of psychological test and objective indicator such as salivary alpha-amylase is an excellent tool for objective evaluation of individual's state in stressful environment. Similar tests may be used in assessment of patients' behaviours at dental treatment that may be considered a stressor in most patients.
唾液α-淀粉酶是一种有用的生物标志物，可用于评估人类心理生物学和社会行为过程。对其进行研究为创建有关生物过程与社会过程相互作用及其对健康和行为影响的新概念开辟了可能性。
对30名年龄为21.37±0.96岁（18名女性和12名男性）的个体进行了两次唾液α-淀粉酶水平测定，并使用斯皮尔伯格测试进行情境焦虑自我评估：一次在压力情境下（考试前），一个月后在无压力环境下（培训期间）再次进行。使用美国Salimetrics LLC的动力学反应试剂盒测量唾液α-淀粉酶。
第一次测量时唾液α-淀粉酶的平均水平为156.0±93.33 U/ml。在第二次无强烈压力的测量中，水平降低了两倍，为74.03±58.06 U/ml，差异具有统计学意义（P<0.001）。我们发现两次测量中唾液α-淀粉酶水平之间存在统计学显著相关性（P<0.01）。相关系数为r = 0.472（P<0.01）。
斯皮尔伯格编制的状态-特质焦虑量表（STAI）修订版适用于评估年轻人与压力相关的焦虑。唾液α-淀粉酶可作为客观评估处于压力环境中个体身心状态的生物标志物。心理测试与唾液α-淀粉酶等客观指标相结合是客观评估个体在压力环境中状态的优秀工具。类似测试可用于评估牙科治疗中患者的行为，而牙科治疗在大多数患者中可能被视为一种压力源。
