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微生物在姜汁发酵过程中对生物医学领域中多酚和黄酮类化合物增加的协同作用。

Synergic Involvements of Microorganisms in the Biomedical Increase of Polyphenols and Flavonoids during the Fermentation of Ginger Juice.

作者信息

Kayath Christian Aimé, Ibala Zamba Armel, Mokémiabeka Saturnin Nicaise, Opa-Iloy Meddy, Elenga Wilson Paola Sandra, Kaya-Ongoto Moïse Doria, Mouellet Maboulou Rodd Jurah, Nguimbi Etienne

机构信息

Laboratoire de Biologie Cellulaire et Moléculaire (BCM), Faculté des Sciences et Techniques, Université Marien Ngouabi, BP. 69, Brazzaville, Congo.

Institut National de Recherche en Sciences Exactes et Naturelles (IRSEN), Avenue de l'Auberge Gascogne, BP. 2400, Brazzaville, Congo.

出版信息

Int J Microbiol. 2020 Aug 1;2020:8417693. doi: 10.1155/2020/8417693. eCollection 2020.

Abstract

Steered fermentation by microorganisms gives great added value in the nutritional quality of local food. Ginger rhizome naturally contains a myriad of bioactive compounds including polyphenol and flavonoids. The aim of this work was to ferment the ginger juice, to evaluate the biochemical parameters of ginger wine, and to understand the involvement of microorganisms in the bioincrease of polyphenol compounds. Titratable acidity and pH values were determined and showed that pH is around 1.6 at the end of the fermentation when the acidity is around 6.431 g/L. Using colorimetric assay, the total polyphenolic and flavonoid compounds were evaluated throughout the fermentation. The variation of the polyphenol and flavonoid concentrations of the unsweetened sample was around 10.18 to 14.64 mg Eq AG/g and 1.394 to 2.224 mg Eq Cat/g Ms, but those from the sweet sample were around 10.82 to 18.34 mg Eq AG/g Ms and 1.311 to 2.290 mg Eq Cat/g. Using one-step PCR, multiplex techniques with specific primers, with yeast-like phenotype 27.27% (6), have been assigned among 22 isolates to By using PCR multiplex techniques, , , , and have been identified. Together with , we showed that sp. are able to secrete enzymatic landscape with some activities up to 50% including cellulase, amylase, pectinase, and protease.

摘要

微生物引导的发酵为当地食物的营养品质带来了巨大的附加值。姜根茎天然含有多种生物活性化合物,包括多酚和黄酮类化合物。这项工作的目的是对姜汁进行发酵,评估姜酒的生化参数,并了解微生物在多酚化合物生物增加中的作用。测定了可滴定酸度和pH值,结果表明在发酵结束时,当酸度约为6.431 g/L时,pH值约为1.6。使用比色法,在整个发酵过程中评估了总多酚和黄酮类化合物。未加糖样品的多酚和黄酮类化合物浓度变化约为10.18至14.64 mg Eq AG/g和1.394至2.224 mg Eq Cat/g Ms,但加糖样品的浓度变化约为10.82至18.34 mg Eq AG/g Ms和1.311至2.290 mg Eq Cat/g。使用一步PCR,通过特异性引物的多重技术,在22株分离物中,具有酵母样表型的27.27%(6株)已被鉴定为 。通过使用PCR多重技术, 、 、 和 已被鉴定。与 一起,我们表明 种能够分泌酶谱,其中一些活性高达50%,包括纤维素酶、淀粉酶、果胶酶和蛋白酶。

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