Okouakoua Frédéric Yannick, Kayath Christian Aimé, Mokemiabeka Saturnin Nicaise, Moukala David Charles Roland, Kaya-Ongoto Moïse Doria, Nguimbi Etienne
Laboratoire de Biologie Cellulaire et Moléculaire (BCM), Faculté des Sciences et Techniques, Université Marien N'GOUABI, BP. 69, Brazzaville, Congo.
Institut National de Recherche en Sciences Exactes et Naturelles (IRSEN), Avenue de l'Auberge Gascogne, B.P 2400, Brazzaville, Congo.
Int J Microbiol. 2024 May 2;2024:6627190. doi: 10.1155/2024/6627190. eCollection 2024.
With species, about 30% of extracellular proteins are translocated through the cytoplasmic membrane, coordinated by the Sec translocase. This system mainly consists of the cytoplasmic ATPase SecA and the membrane-embedded SecYEG channel. The purpose of this work was to investigate the effects of the SecYEG export system on the production of industrial biomolecules, such as biosurfactants, proteases, amylases, and cellulases. Fifty-two isolates of species were obtained from traditional fermented foods and then characterized using molecular microbiology methods. The isolates secreted exoenzymes that included cellulases, amylases, and proteases. We present evidence that a biosurfactant-like molecule requires the SecA ATPase and the SecYEG membrane channel for its secretion. In addition, we showed that biomolecules involved in biofilm formation required the SecYEG pathway. This work presents a novel seven-target fragment multiplex PCR assay capable of identification at the species level of through a unique SecDF chromosomal gene. The bacterial membrane protein SecDF allowed the discrimination of , , and . SecA was able to interact with AprE, AmyE, and TasA. The Rose Bengal inhibitor of SecA crucially affected the interaction of AprE, AmyE, TapA, and TasA with recombinant Gst-SecA. The Rose Bengal prevented species from secreting and producing proteases, cellulases, amylases, and biosurfactant-like molecules. It also inhibited the formation of biofilm cell communities. The data support, for the first time, that the SecYEG translocon mediates the secretion of a biosurfactant-like molecule.
对于某些物种而言，约30%的细胞外蛋白通过细胞质膜转运，这一过程由Sec转运酶协调。该系统主要由细胞质ATP酶SecA和嵌入膜中的SecYEG通道组成。本研究的目的是探究SecYEG输出系统对工业生物分子（如生物表面活性剂、蛋白酶、淀粉酶和纤维素酶）生产的影响。从传统发酵食品中获得了52株该物种的分离株，然后使用分子微生物学方法对其进行表征。这些分离株分泌包括纤维素酶、淀粉酶和蛋白酶在内的胞外酶。我们提供的证据表明，一种类似生物表面活性剂的分子的分泌需要SecA ATP酶和SecYEG膜通道。此外，我们还表明，参与生物膜形成的生物分子需要SecYEG途径。本研究提出了一种新型的七靶点片段多重PCR检测方法，该方法能够通过独特的SecDF染色体基因在物种水平上鉴定该物种。细菌膜蛋白SecDF能够区分该物种、另一物种、又一物种和再一物种。SecA能够与AprE、AmyE和TasA相互作用。孟加拉玫瑰红对SecA的抑制作用严重影响了AprE、AmyE、TapA和TasA与重组Gst-SecA的相互作用。孟加拉玫瑰红阻止该物种分泌和产生蛋白酶、纤维素酶、淀粉酶和类似生物表面活性剂的分子。它还抑制生物膜细胞群落的形成。这些数据首次支持SecYEG转运体介导一种类似生物表面活性剂分子的分泌。
