Association of specific microorganisms with endodontic signs and symptoms. A comparative study.

AIM

The study aimed to evaluate the association of root canal microorganisms red complex and with endodontic clinical signs and symptoms using polymerase chain reaction.

MATERIALS AND METHODS

Bacterial samples were obtained using sterile paper points from the teeth of 100 subjects divided into two groups; Group I: 50 individuals with primary tooth infections and Group II: 50 individuals with failed endodontic treatment having the secondary infection. DNA extracted from samples was analyzed for endodontic pathogens by using species-specific primers.

RESULTS

The pain was noticed in 66%, (33 of 50 subjects) in primary infection and 60% (30 of 50) in a secondary infection. A statistically significant association between pain and bacteria observed both in primary infection and secondary infection ( < 0.05). Tenderness on percussion was associated with 40% cases in Group I and 70% cases in Group II. The red complex accounted for 94% of cases associated with tenderness on percussion in primary infection while 86% of cases associated with secondary infection with a statistically significant association ( < 0.05).

CONCLUSION

Prevalence of red complex bacteria and suggested the association of studied bacteria with symptomatic infected pulp and periradicular diseases.