Tarazón Estefanía, Portolés Manuel, Roselló-Lletí Esther
Myocardial Dysfunction and Cardiac Transplantation Group and CIBERCV, Health Research Institute of La Fe University Hospital (IIS La Fe), Valencia 46026, Spain.
STAR Protoc. 2020 Sep 10;1(2):100100. doi: 10.1016/j.xpro.2020.100100. eCollection 2020 Sep 18.
Detailed study of cellular organelles requires their isolation. Several protocols have been described for the isolation of the Golgi apparatus from liver tissue, but these are not suitable and not reproducible in harder tissues. Here, we describe a protocol to isolate Golgi vesicles from cardiac tissue using a discontinuous sucrose gradient. For complete details on the use and execution of this protocol, please refer to Tarazon et al. (2017).
对细胞器进行详细研究需要将它们分离出来。已经有几种从肝脏组织中分离高尔基体的方案，但这些方案在较硬的组织中并不适用且无法重复。在此，我们描述一种使用不连续蔗糖梯度从心脏组织中分离高尔基体囊泡的方案。有关该方案使用和实施的完整详细信息，请参考塔拉松等人（2017年）的研究。
