Oldoni Tatiane L C, Silva Rafaela C, Carpes Solange T, Massarioli Adna P, Alencar Severino M DE
Universidade Tecnológica Federal do Paraná/UTFPR, Departamento de Química, Via do Conhecimento, Km 01, 85503-390 Pato Branco, PR, Brazil.
Universidade de São Paulo/USP, Departamento de Agroindústria, Alimentos e Nutrição, Escola Superior de Agricultura Luiz de Queiroz, Avenida Pádua Dias, 11, 13418-900 Piracicaba, SP, Brazil.
An Acad Bras Cienc. 2020 Oct 23;92(3):e20181068. doi: 10.1590/0001-3765202020181068. eCollection 2020.
A chromatographic method consisting of multi wavelength detection for identification of six phenolic acids, one stilbene and five flavonoids in grape and apple pomaces was proposed. Scavenging of DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), reactive oxygen species and reduction of Fe3+ to Fe2+ using in vitro and HPLC-UV-ABTS on-line methods are herein presented. A reversed phase C18 coupled with an absorption detector operating at 280, 300, 320 and 360 nm for the benzoic acid derivatives and flavanols; stilbenes; cinnamic acid derivatives and flavonols, were respecctively used. The solvents water, methanol and acetonitrile acidified with acetic acid were evaluated as mobile phase. The optimized chromatographic method presented recoveries ranged from 68 to 130% and from 66 to 130% for grape and apple pomaces respectively. The determination coefficients (R2) of the 12 compounds were > 0.98. The extracts showed high total phenolic content and exhibits strong capacities to scavenge free radicals and reactive oxygen species. The results obtained by HPLC-ABTS on-line method suggest that pomaces of grape and apple are rich in bioactive compounds and that catechin and epicatechin contribute in a significantly way to the antioxidant activity in both agroindustrial pomaces.
提出了一种用于鉴定葡萄和苹果渣中六种酚酸、一种芪类化合物和五种黄酮类化合物的多波长检测色谱方法。本文介绍了使用体外和HPLC-UV-ABTS在线方法清除DPPH（2,2-二苯基-1-苦基肼）、ABTS（2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）、活性氧以及将Fe3+还原为Fe2+的情况。使用反相C18柱，分别与在280、300、320和360nm波长下运行的吸收检测器联用，用于检测苯甲酸衍生物和黄烷醇、芪类化合物、肉桂酸衍生物和黄酮醇。评估了用乙酸酸化的水、甲醇和乙腈作为流动相。优化后的色谱方法对葡萄渣和苹果渣的回收率分别为68%至130%和66%至130%。12种化合物的测定系数（R2）>0.98。提取物显示出高总酚含量，并具有很强的清除自由基和活性氧的能力。通过HPLC-ABTS在线方法获得的结果表明，葡萄和苹果的果渣富含生物活性化合物，儿茶素和表儿茶素对这两种农业工业果渣的抗氧化活性有显著贡献。
