Graduate Program in Health and Development in the Central-West Region of Brazil, Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil.
Group of Spectroscopy and Bioinformatics Applied Biodiversity and Health (GEBABS), Federal University of Mato Grosso do Sul, Campo Grande 79070-900, Brazil.
Molecules. 2022 Jun 1;27(11):3563. doi: 10.3390/molecules27113563.
Antioxidants have drawn the attention of the scientific community due to being related to the prevention of various degenerative diseases. The antioxidant capacity has been extensively studied in vitro, and different methods have been used to assess its activity. However, the main issues related to studying natural antioxidants are evaluating whether these antioxidants demonstrate a key role in the biological system and assessing their bioavailability in the organism. The majority of outcomes in the literature are controversial due to a lack of method standardization and their proper application. Therefore, this study aims to compile the main issues concerning the natural antioxidant field of study, comparing the most common in vitro methods to evaluate the antioxidant activity of natural compounds, demonstrating the antioxidant activity in biological systems and the role of the main antioxidant enzymes of redox cellular signaling and explaining how the bioavailability of bioactive compounds is evaluated in animal models and human clinical trials.
抗氧化剂因其与预防各种退行性疾病有关而引起了科学界的关注。抗氧化能力已在体外得到广泛研究，并且已经使用了不同的方法来评估其活性。然而，研究天然抗氧化剂的主要问题是评估这些抗氧化剂是否在生物系统中发挥关键作用，以及评估它们在生物体中的生物利用度。由于缺乏方法标准化及其正确应用，文献中的大多数结果存在争议。因此，本研究旨在综述天然抗氧化剂研究领域的主要问题，比较评估天然化合物抗氧化活性的最常见体外方法，证明生物系统中的抗氧化活性以及细胞信号转导中主要抗氧化酶的作用，并解释如何在动物模型和人体临床试验中评估生物活性化合物的生物利用度。
