College of Life Science and Engineering, Shenyang University, Dadong District, Shenyang City, Liaoning Province, People's Republic of China.
J Med Entomol. 2021 Mar 12;58(2):616-624. doi: 10.1093/jme/tjaa219.
Dohrniphora cornuta (Bigot) is a forensically important phorid fly indoors and in burial environments. The determination of a minimum postmortem interval (PMImin) often relies on the determination of the age of the immatures. Although the larval development data of D. cornuta under different temperatures has been established, the intrapuparial stage which lasts for about half of the total immature development is scarce. In this study, we investigated the key morphological changes during intrapuparial development at constant temperatures (15, 18, 21, 24, 27, 30, 33, and 36°C), with an aim to estimate the intrapuparial age of D. cornuta. Puparia were sampled at 12-h (24, 27, 30, and 33°C), 24-h (18 and 21°C), and 48-h (15°C) intervals. The morphological developments within the puparium were analyzed using a stereomicroscope after the puparium was removed. The average minimum duration of intrapuparial stage was inversely related to temperature, ranging from 184.79 ± 3.00 h at 30°C to 1102.86 ± 25.55 h at 15°C for female, and 197.40 ± 4.12 h at 30°C to 1175.33 ± 18.55 h at 15°C for male. It did not develop at 36°C. Some morphological traits that changed during development within the puparium could be used as age markers. According to these changes, the intrapuparial stage of D. cornuta was divided into nine stages which could be used for both sexes. This study provides relatively systematic development data of D. cornuta intrapuparial for the estimation of PMImin in forensic entomology.
角蝉蛹（Bigot）是室内和埋葬环境中一种具有重要法医学意义的腐生性蝇类。死后时间推断（PMImin）的确定通常依赖于对幼期的年龄测定。尽管已经建立了角蝉蛹在不同温度下的幼虫发育数据，但持续约整个幼期一半时间的蛹内期却很少有研究。在本研究中，我们在恒温（15、18、21、24、27、30、33 和 36°C）下研究了蛹内发育过程中的关键形态变化，旨在估算角蝉蛹的蛹内期年龄。每隔 12 小时（24、27 和 33°C）、24 小时（18 和 21°C）和 48 小时（15°C）采集蛹样本。在去除蛹后，使用立体显微镜分析蛹内的形态发育。雌性的平均最小蛹内期持续时间与温度呈反比，范围从 30°C 的 184.79±3.00 小时到 15°C 的 1102.86±25.55 小时，雄性的平均最小蛹内期持续时间与温度呈反比，范围从 30°C 的 197.40±4.12 小时到 15°C 的 1175.33±18.55 小时。36°C 时则不会发育。在蛹内发育过程中发生变化的一些形态特征可用作年龄标记。根据这些变化，将角蝉蛹的蛹内期分为九个阶段，可用于雌雄两性。本研究为法医昆虫学中估计 PMImin 提供了相对系统的角蝉蛹蛹内发育数据。
