Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, Shenyang University, Shenyang 110044, China.
Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, Shenyang University, Shenyang 110044, China.
Forensic Sci Int. 2014 Mar;236:133-7. doi: 10.1016/j.forsciint.2014.01.002. Epub 2014 Jan 13.
In addition to causing myiasis in humans and animals, Megaselia scalaris (Loew) has been reported as a forensically important fly. The determination of a minimum postmortem interval (PMI) often relies on the determination of the age of the larvae and pupae. The pupal stage represents about 50% of the immature development time and the pupal age may therefore serve as an important tool in entomological minimum PMI estimation. The present study focuses on the key developmental processes during metamorphosis of M. scalaris pupae at different constant temperatures (18, 21, 24, 27, 30, 33 and 36°C). The average minimum duration of development from prepupa to adult emergence was inversely related to temperature, ranging from 170.65 ± 1.39 h at 33°C to 608.80 ± 13.26 h at 18°C. The pupa did not develop at 36°C. Some morphological features that changed during development within the puparium could be used as age markers. According to these changes, the pupal stage of M. scalaris was divided into 10 stages which could be used for both sexes.
此外，斯氏巨蚤（Loew）除了在人类和动物中引起蝇蛆病外，还被报道为一种具有法医学重要性的蝇类。确定最小死后间隔时间（PMI）通常依赖于幼虫和蛹的年龄确定。蛹期约占未成熟发育时间的 50%，因此蛹龄可以作为昆虫学 PMI 估计的重要工具。本研究重点关注不同恒温（18、21、24、27、30、33 和 36°C）下斯氏巨蚤蛹变态过程中的关键发育过程。从预蛹到成虫出现的平均最短发育时间与温度呈反比，在 33°C 时为 170.65±1.39 h，在 18°C 时为 608.80±13.26 h。36°C 时蛹不发育。在蛹壳内发育过程中发生变化的一些形态特征可用作年龄标记。根据这些变化，斯氏巨蚤的蛹期被分为 10 个阶段，可用于雌雄两性。
