Calcium signaling in endocardial and epicardial ventricular myocytes from streptozotocin-induced diabetic rats.

AIMS/INTRODUCTION: Abnormalities in Ca signaling have a key role in hemodynamic dysfunction in diabetic heart. The purpose of this study was to explore the effects of streptozotocin (STZ)-induced diabetes on Ca signaling in epicardial (EPI) and endocardial (ENDO) cells of the left ventricle after 5-6 months of STZ injection.

MATERIALS AND METHODS

Whole-cell patch clamp was used to measure the L-type Ca channel (LTCC) and Na /Ca exchanger currents. Fluorescence photometry techniques were used to measure intracellular free Ca concentration.

RESULTS

Although the LTCC current was not significantly altered, the amplitude of Ca transients increased significantly in EPI-STZ and ENDO-STZ compared with controls. Time to peak LTCC current, time to peak Ca transient, time to half decay of LTCC current and time to half decay of Ca transients were not significantly changed in EPI-STZ and ENDO-STZ myocytes compared with controls. The Na /Ca exchanger current was significantly smaller in EPI-STZ and in ENDO-STZ compared with controls.

CONCLUSIONS

STZ-induced diabetes resulted in an increase in amplitude of Ca transients in EPI and ENDO myocytes that was independent of the LTCC current. Such an effect can be attributed, at least in part, to the dysfunction of the Na /Ca exchanger. Additional studies are warranted to improve our understanding of the regional impact of diabetes on Ca signaling, which will facilitate the discovery of new targeted treatments for diabetic cardiomyopathy.