Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California, USA.
Department of Nutrition and Food Safety, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Foodborne Pathog Dis. 2020 Dec;17(12):739-742. doi: 10.1089/fpd.2020.2792. Epub 2020 Oct 28.
Thirty-four isolates from 91 ready-to-eat lettuce packages, obtained from local supermarkets in Northern California, were genotyped by multilocus sequence typing, tested for susceptibility to antimicrobial agents, and screened for β-lactamase genes. We found 15 distinct sequence types (STs). Six of these genotypes (ST1198, ST2625, ST2432, ST2819, ST4600, and ST5143) have been reported as pathogens found in human samples. Twenty-six (76%) isolates were resistant to ampicillin, 17 (50%) to ampicillin/sulbactam, 8 (23%) to cefoxitin, and 7 (20%) to cefuroxime. was the most prevalent β-lactamase gene, identified in eight (23%) isolates. We identified a class A broad-spectrum β-lactamase SED-1 gene, , reported by others in isolated from bile of a patient. This study found that fresh lettuce carries β-lactam drug-resistant , which might serve as a reservoir for drug-resistance genes that could potentially be transmitted to pathogens that cause human infections.
34 株从加利福尼亚州北部当地超市购买的即食生菜包中分离得到的 91 株分离株，通过多位点序列分型进行了基因分型，对其对抗菌药物的敏感性进行了检测，并对β-内酰胺酶基因进行了筛查。我们发现了 15 个不同的序列类型（ST）。其中 6 种基因型（ST1198、ST2625、ST2432、ST2819、ST4600 和 ST5143）已被报道为在人类样本中发现的病原体。26 株（76%）对氨苄西林耐药，17 株（50%）对氨苄西林/舒巴坦耐药，8 株（23%）对头孢西丁耐药，7 株（20%）对头孢呋辛耐药。是最常见的β-内酰胺酶基因，在 8 株（23%）分离株中发现。我们鉴定出一种 A 类广谱β-内酰胺酶 SED-1 基因，其他人曾在从一名患者胆汁中分离到的 中报道过该基因。本研究发现，新鲜生菜携带耐β-内酰胺药物的 ，可能成为耐药基因的储存库，这些耐药基因可能会传播给导致人类感染的病原体。
