Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin 130000, China.
Epigenomics. 2020 Oct;12(20):1811-1824. doi: 10.2217/epi-2020-0125. Epub 2020 Oct 28.
To study RNA N6-methyladenosine (m6A) modification in the diabetic hippocampus. Behavioral tests and staining were performed to evaluate the damage to the diabetic hippocampus in model rats. Western blotting was performed to investigate the expression of methylation-related enzymes, and flow cytometry was used to demonstrate HT22 cell apoptosis. M6A and RNA sequencing analyses were conducted to profile m6A-tagged transcripts in the diabetic hippocampus. The rat models of diabetes mellitus suffered from cognitive disorders and hippocampal neuron damage. High glucose levels altered the expression of methylation-related enzymes. A total of 4890 differentially methylated m6A peaks and 63 differentially expressed genes and differentially methylated m6A sites were identified. The findings suggest that m6A modification is altered in the diabetic hippocampus and provide new insight into diabetic hippocampal injury.
研究糖尿病海马中的 RNA N6-甲基腺苷（m6A）修饰。通过行为学测试和染色来评估糖尿病模型大鼠海马损伤情况。采用 Western blot 法检测甲基化相关酶的表达，流式细胞术检测 HT22 细胞凋亡。通过 m6A 测序分析来描绘糖尿病海马中的 m6A 修饰转录本。
糖尿病模型大鼠表现出认知障碍和海马神经元损伤。高糖水平改变了甲基化相关酶的表达。共鉴定出 4890 个差异甲基化 m6A 峰和 63 个差异表达基因和差异甲基化 m6A 位点。
糖尿病海马中 m6A 修饰发生改变，为糖尿病海马损伤提供了新的见解。
