The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, People's Republic of China.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, People's Republic of China.
Arch Microbiol. 2021 Apr;203(3):1001-1008. doi: 10.1007/s00203-020-02091-8. Epub 2020 Oct 28.
Germplasm of industrial brewing yeast of the worldwide have a richer diversity, and various successes in improving the performance of brewing yeasts. However, they are limited in that they have relatively low odds of combining desirable traits in a correct manner. To improve germplasm resource preservation, management, and utilization efficiency. In this study, the genetic diversity of 35 industrial brewing yeasts were analyzed based upon inter simple sequence repeat (ISSR) markers, in which 151 out of 167 SSR loci (90.42%) were polymorphic between two or more strains. Three preliminary core collections were established using ISSR data, and based on three different strategies as follows: an advanced maximization (M) strategy, an allele preferred sampling (A) strategy, and a random sampling (R) strategy. Comparison of genetic parameters, including polymorphic information content, Nei's genetic diversity (H), effective allele number, observed allele number, Shannon's index (I), and principal coordinate analyses, confirmed that all the core collections accurately recapitulated the diversity of the initial germplasm. Considering the loci retention ratio and trait coverage efficiency, Core1 was considered the best core collection.
工业酿造酵母的种质资源在全球范围内具有更丰富的多样性，并在提高酿造酵母性能方面取得了各种成功。然而，它们的局限性在于，以正确的方式组合理想特性的可能性相对较低。为了提高种质资源的保存、管理和利用效率。在这项研究中，基于简单序列重复（SSR）标记分析了 35 株工业酿造酵母的遗传多样性，其中 167 个 SSR 位点数（90.42%）中有 151 个在两个或更多菌株之间具有多态性。使用 ISSR 数据建立了三个初步的核心群体，基于以下三种不同的策略：先进的最大化（M）策略、等位基因偏好采样（A）策略和随机采样（R）策略。遗传参数的比较，包括多态信息量、Nei 的遗传多样性（H）、有效等位基因数、观察等位基因数、Shannon 指数（I）和主坐标分析，证实所有核心群体都准确地概括了初始种质资源的多样性。考虑到位点保留率和性状覆盖效率，Core1 被认为是最佳核心群体。
