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一种SNP分子鉴定系统在……中的建立与应用 （原文句子不完整，翻译可能不太准确，建议补充完整原文）

Establishment and application of a SNP molecular identification system in .

作者信息

Diao Bin, Xu Zhixiang, Liu Min, Zhang Guoli, Wang Guangyuan, Zhang Yinghao, Tian Xuemei

机构信息

Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University, Qingdao, China.

出版信息

Front Microbiol. 2024 Aug 8;15:1417014. doi: 10.3389/fmicb.2024.1417014. eCollection 2024.

DOI:10.3389/fmicb.2024.1417014
PMID:39176275
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11340509/
Abstract

Germplasm resources of edible mushrooms are essential for the breeding of varieties with improved traits. Analysis of the genetic diversity of germplasm resources and clarification of the genetic relationships among strains can provide valuable information for the selection of breeding parents. A total of 829,488 high-quality SNP loci were screened from 2,125,382 SNPs obtained by sequencing 60 . Phylogenetic analysis, PCA, and population structure analysis based on the high-quality SNPs showed that the 60 strains could be divided into five subgroups, and the clustering results were consistent with the geographical distributions of these strains. Based on high-quality SNP loci, a core collection containing 18 representative germplasm resources was constructed, and 1,473 Kompetitive Allele-Specific PCR markers were obtained. A total of 722 SNP markers in the exonic regions were screened using KASP-genotyping experiments, and 50 candidate SNP markers and 12 core SNP markers were obtained. Genetic fingerprints of germplasm resources were constructed based on the selected SNP markers; these fingerprints provide an accurate, rapid, convenient, and efficient method for the identification of germplasm resources. The results of this study have important implications for the preservation and utilization of germplasm resources and the identification of varieties.

摘要

食用菌种质资源对于培育性状改良品种至关重要。分析种质资源的遗传多样性并阐明菌株间的遗传关系可为育种亲本的选择提供有价值的信息。从对60个样本测序获得的2,125,382个单核苷酸多态性（SNP）中筛选出了总共829,488个高质量SNP位点。基于这些高质量SNP进行的系统发育分析、主成分分析（PCA）和群体结构分析表明，这60个菌株可分为五个亚组，聚类结果与这些菌株的地理分布一致。基于高质量SNP位点，构建了包含18份代表性种质资源的核心种质库，并获得了1,473个竞争性等位基因特异性PCR标记。通过竞争性等位基因特异性PCR（KASP）基因分型实验筛选出了外显子区域的722个SNP标记，获得了50个候选SNP标记和12个核心SNP标记。基于所选SNP标记构建了种质资源的遗传指纹图谱；这些指纹图谱为种质资源的鉴定提供了一种准确、快速、便捷且高效的方法。本研究结果对种质资源的保存与利用以及品种鉴定具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/b07fca27a1ab/fmicb-15-1417014-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/32e27992476a/fmicb-15-1417014-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/16340a4a4a3d/fmicb-15-1417014-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/ac289a4fd722/fmicb-15-1417014-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/8be77fe20b8a/fmicb-15-1417014-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/5d91cd6cbe44/fmicb-15-1417014-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/b07fca27a1ab/fmicb-15-1417014-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/32e27992476a/fmicb-15-1417014-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/16340a4a4a3d/fmicb-15-1417014-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/ac289a4fd722/fmicb-15-1417014-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/8be77fe20b8a/fmicb-15-1417014-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/5d91cd6cbe44/fmicb-15-1417014-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8388/11340509/b07fca27a1ab/fmicb-15-1417014-g006.jpg

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