Department of General Surgery, Sixth Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.
Department of Medical Center, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
J Fluoresc. 2021 Jan;31(1):119-127. doi: 10.1007/s10895-020-02636-6. Epub 2020 Oct 28.
In this study, a new Cd(II)-bearing coordination polymer with the chemical formula of {[Cd(meda)(dpe)(HO)]·(NO)·2(HO)} (1, Hmeda = 3,3'-methylenedibenzoic acid, dpe = 1,2-di(pyridin-4-yl)ethane) has been successfully prepared by reaction of Cd(NO)·4HO with a V-shape carboxyl ligand Hmeda along with the linear dipyridine ligand dpe under the hydrothermal conditions. Due to its intensive luminescence, complex 1 could be utilized as the sensor of detecting Al ion, and its detection limit is 4 × 10 M. Firstly, the toxicity of the compound on the normal liver cells was determined with Cell Counting Kit-8 detection kit. The triglyceride in liver cells was detected by detection kit after compound treatment and the relative expression of 15-lox and 12-lox in L02 cells was also measured by RT-PCR after compound treatment. In addition, multiple functional groups that provided by the synthesized Cd(II) complex have been studied by using molecular docking simulation for the confirmation of possible binding modes that formed between ligand and receptor.
在这项研究中，通过将 Cd(NO)·4HO 与 V 形羧酸配体 Hmeda 以及线性二吡啶配体 dpe 在水热条件下反应，成功制备了化学式为{[Cd(meda)(dpe)(HO)]·(NO)·2(HO)}的新型含 Cd(II)配位聚合物（1，Hmeda=3,3'-亚甲基二苯甲酸，dpe=1,2-二（吡啶-4-基）乙烷）。由于其强烈的发光性质，配合物 1 可被用作检测 Al 离子的传感器，其检测限为 4×10−5 M。首先，使用细胞计数试剂盒-8 检测试剂盒测定了化合物对正常肝细胞的毒性。用检测试剂盒检测化合物处理后肝细胞中的甘油三酯，并用 RT-PCR 测定化合物处理后 L02 细胞中 15-lox 和 12-lox 的相对表达量。此外，还通过分子对接模拟研究了合成的 Cd(II)配合物中提供的多种功能基团，以确认配体与受体之间形成的可能结合模式。
