Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Department of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.
J Fluoresc. 2021 Sep;31(5):1343-1352. doi: 10.1007/s10895-021-02768-3. Epub 2021 Jun 15.
In the current study, on the basis of 1,3,5-tris(2-methylimidazol-1-yl)benzene (timb), a designed tripodal connector, two new transition metal coordination polymers (CPs), {[Cu(timb)(Br-IPA)]·5HO} (1) and {[Zn(timb)(NH-IPA)]·4HO} (2) have been generated with the mixed ligand method by the reaction between the timb and corresponding metal salts in the existence of dissimilar functional isophthalic acid (HIPA) ligands. Furthermore, the Zn(II)-based complex 2 displays high sensitivity in the detection of Cu(II) ion in water. The neural stem cells proliferation after treated via compounds was detected with Cell Counting Kit-8 detection assay. And the real time reverse transcription polymerase chain reaction was carried out for the investigation of the differentiation function of the neural stem cells after the compound 1 treatment and compound 2 treatment. Further, molecular docking simulations confirmed that the biological activity that has been observed from experiments were from the carboxyl group on the Cu complex, in contrast, the imidazole groups were only used for binding with the Cu metal ion to retain the complex structure.
在本研究中，基于设计的三齿连接体 1,3,5-三(2-甲基咪唑-1-基)苯（timb），通过 timb 与相应金属盐在不同功能的间苯二甲酸（HIPA）配体存在下的反应，采用混合配体法生成了两个新的过渡金属配位聚合物（CPs）：{[Cu(timb)(Br-IPA)]·5H2O}（1）和{[Zn(timb)(NH-IPA)]·4H2O}（2）。此外，基于 Zn(II)的配合物 2 在水中对 Cu(II)离子具有高灵敏度的检测性能。通过 Cell Counting Kit-8 检测试剂盒检测化合物处理后的神经干细胞增殖情况。并且通过实时逆转录聚合酶链反应（qRT-PCR）研究了化合物 1 和化合物 2 处理后神经干细胞的分化功能。进一步的分子对接模拟证实，从实验中观察到的生物活性来自于 Cu 配合物上的羧基，而咪唑基仅用于与 Cu 金属离子结合以保持配合物的结构。
