Ikeda Miho, Takahashi Mikiya, Fujiwara Sumire, Mitsuda Nobutaka, Ohme-Takagi Masaru
Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba 305-8566, Japan.
Plants (Basel). 2020 Oct 25;9(11):1434. doi: 10.3390/plants9111434.
The induction of adventitious organs, such as calli, shoots, and somatic embryos, in tissue culture is a useful technique for plant propagation and genetic modification. In recent years, several genes have been reported to be adventitious organ inducers and proposed to be useful for industrial applications. Even though the Arabidopsis () () and () genes can induce adventitious organ formation in Arabidopsis without phytohormone treatment, further improvement is desired. Here, we show that modifying the transcriptional repression/activation activities of WUS and LEC1 improves the efficiency of adventitious organ formation in Arabidopsis. Because WUS functions as a transcriptional repressor during the induction of adventitious organs, we fused it to an artificial strong repression domain, SUPERMAN REPRESSION DOMAIN X (SRDX). Conversely, we fused the strong transcriptional activation domain VP16 from herpes simplex virus to LEC1. Upon overexpression of the corresponding transgenes, we succeeded in improving the efficiency of adventitious organ induction. Our results show that the modification of transcriptional repression/activation activity offers an effective method to improve the efficiency of adventitious organ formation in plants.
在组织培养中诱导不定器官（如愈伤组织、芽和体细胞胚）是植物繁殖和基因改造的一项有用技术。近年来，已有多个基因被报道为不定器官诱导基因，并被认为可用于工业应用。尽管拟南芥的WUS、LEC1和STM基因在无需植物激素处理的情况下就能诱导拟南芥不定器官的形成，但仍需要进一步改进。在此，我们表明，改变WUS和LEC1的转录抑制/激活活性可提高拟南芥不定器官形成的效率。由于WUS在不定器官诱导过程中起转录抑制因子的作用，我们将其与人工强抑制结构域——超man抑制结构域X（SRDX）融合。相反，我们将单纯疱疹病毒的强转录激活结构域VP16与LEC1融合。在相应转基因过表达后，我们成功提高了不定器官诱导的效率。我们的结果表明，转录抑制/激活活性的改变为提高植物不定器官形成效率提供了一种有效方法。
