González Julia, González Marta G, Valcárcel Félix, Sánchez María, Martín-Hernández Raquel, Tercero José M, Olmeda A Sonia
Villamagna S.A., Finca ''La Garganta'', 14440 Villanueva de Córdoba, Spain.
Center for Vector Biology, Department of Entomology, Rutgers University, New Brunswick, NJ 08901, USA.
Pathogens. 2020 Oct 25;9(11):884. doi: 10.3390/pathogens9110884.
(Derrick) Philip, the causative agent of Q fever, is mainly transmitted by aerosols, but ticks can also be a source of infection. Transstadial and transovarial transmission of by (Koch) has been suggested. There is a close relationship between this tick species, wild animals and but the transmission in a natural environment has not been demonstrated. In this study, we collected 80 engorged nymphs of from red deer and wild rabbits. They moult to adults under laboratory conditions and we feed them artificially through silicone membranes after a preconditioning period. DNA was tested in ticks, blood and faeces samples using real-time PCR. The pathogen was found in 36.2% of fed adults, demonstrating that transstadial transmission from nymph to adult occurs in nature. The presence of DNA in the 60.0% of blood samples after artificial feeding confirms that adults transmit the bacteria during feeding. Further studies are needed about co-feeding and other possible transmission routes to define the role of this tick species in the cycle of .
（德里克）Q热的病原体伯氏考克斯体主要通过气溶胶传播，但蜱虫也可能是感染源。有人提出伯氏考克斯体可经蜱的经变态期和经卵传播（科赫）。这种蜱虫物种、野生动物与伯氏考克斯体之间存在密切关系，但在自然环境中的传播尚未得到证实。在本研究中，我们从马鹿和野兔身上采集了80只饱血的幼蜱。它们在实验室条件下蜕皮为成虫，经过预处理期后，我们通过硅胶膜人工喂养它们。使用实时PCR检测蜱虫、血液和粪便样本中的伯氏考克斯体DNA。在36.2%的饱血成虫中发现了病原体，表明从若虫到成虫的经变态期传播在自然环境中会发生。人工喂养后60.0%的血液样本中存在DNA，证实成虫在进食期间会传播细菌。需要进一步研究共进食和其他可能的传播途径，以确定这种蜱虫物种在伯氏考克斯体传播循环中的作用。
