Stewart C, Burke B
European Molecular Biology Laboratory, Heidelberg Federal Republic of Germany.
Cell. 1987 Nov 6;51(3):383-92. doi: 10.1016/0092-8674(87)90634-9.
The nuclear lamina in adult mammalian somatic cells is composed of three major proteins, lamins A, B, and C. The expression of these proteins during the differentiation of teratocarcinomas and mouse embryogenesis is described. Embryos up to day 8 of gestation and embryonal carcinoma (EC) cells express only a single lamin species closely resembling, if not identical to, lamin B. Lamins A and/or C were detected in fertilized eggs, but disappear during the first 2-4 cleavage divisions, only reappearing in 8 day post-implantation embryos. These two lamins are absent from EC cells, but are strongly expressed in some of their derivatives. These results show that cells of the early mouse embryo do not have a functional requirement for lamins A and C and imply that the structural organization of the nucleus may change fundamentally during embryogenesis.
成年哺乳动物体细胞中的核纤层由三种主要蛋白质组成，即核纤层蛋白A、B和C。本文描述了这些蛋白质在畸胎瘤分化和小鼠胚胎发育过程中的表达情况。妊娠8天以内的胚胎和胚胎癌细胞仅表达一种与核纤层蛋白B极为相似（即便不是完全相同）的核纤层蛋白。在受精卵中可检测到核纤层蛋白A和/或C，但在最初的2 - 4次卵裂过程中消失，直到植入后8天的胚胎中才再次出现。这两种核纤层蛋白在胚胎癌细胞中不存在，但在其一些衍生物中强烈表达。这些结果表明，早期小鼠胚胎细胞对核纤层蛋白A和C没有功能需求，这意味着细胞核的结构组织在胚胎发育过程中可能会发生根本性变化。
