快速眼动睡眠剥夺后Wistar大鼠脑和肝组织中的差异基因表达

Differential Gene Expression in Brain and Liver Tissue of Wistar Rats after Rapid Eye Movement Sleep Deprivation.

作者信息

Pandey Atul, Oliver Ryan, Kar Santosh K

机构信息

School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India.

Department of Ecology, Evolution, and Behavior, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

出版信息

Clocks Sleep. 2020 Oct 23;2(4):442-465. doi: 10.3390/clockssleep2040033.

DOI:10.3390/clockssleep2040033

PMID:33114225

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7711450/

Abstract

Sleep is essential for the survival of most living beings. Numerous researchers have identified a series of genes that are thought to regulate "sleep-state" or the "deprived state". As sleep has a significant effect on physiology, we believe that lack of total sleep, or particularly rapid eye movement (REM) sleep, for a prolonged period would have a profound impact on various body tissues. Therefore, using the microarray method, we sought to determine which genes and processes are affected in the brain and liver of rats following nine days of REM sleep deprivation. Our findings showed that REM sleep deprivation affected a total of 652 genes in the brain and 426 genes in the liver. Only 23 genes were affected commonly, 10 oppositely, and 13 similarly across brain and liver tissue. Our results suggest that nine-day REM sleep deprivation differentially affects genes and processes in the brain and liver of rats.

摘要

睡眠对大多数生物的生存至关重要。众多研究人员已经鉴定出一系列被认为可调节“睡眠状态”或“剥夺状态”的基因。由于睡眠对生理机能有重大影响，我们认为长期缺乏充足睡眠，尤其是快速眼动（REM）睡眠，会对身体的各种组织产生深远影响。因此，我们采用微阵列方法，试图确定在大鼠经历九天的REM睡眠剥夺后，大脑和肝脏中哪些基因及过程会受到影响。我们的研究结果表明，REM睡眠剥夺共影响了大脑中的652个基因和肝脏中的426个基因。大脑和肝脏组织中仅有23个基因受到共同影响，10个基因受到相反影响，13个基因受到相似影响。我们的结果表明，九天的REM睡眠剥夺对大鼠大脑和肝脏中的基因及过程产生了不同的影响。