Go Eun-Jin, Yang Hannah, Chon Hong Jae, Yang DaSom, Ryu WonHyoung, Kim Dong-Hyun, Han Dong Keun, Kim Chan, Park Wooram
Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-Si, Gyeonggi-do 14662, Korea.
Department of Biomedical Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13488, Korea.
Cancers (Basel). 2020 Oct 26;12(11):3123. doi: 10.3390/cancers12113123.
Recently, cancer immunotherapy has received attention as a viable solution for the treatment of refractory tumors. However, it still has clinical limitations in its treatment efficacy due to inter-patient tumor heterogeneity and immunosuppressive tumor microenvironment (TME). In this study, we demonstrated the triggering of anti-cancer immune responses by a combination of irreversible electroporation (IRE) and a stimulator of interferon genes (STING) agonist. Optimal electrical conditions inducing damage-associated molecular patterns (DAMPs) by immunogenic cell death (ICD) were determined through in vitro 2D and 3D cell experiments. In the in vivo syngeneic lung cancer model, the combination of IRE and STING agonists demonstrated significant tumor growth inhibition. We believe that the combination strategy of IRE and STING agonists has potential for effective cancer immunotherapy.
最近，癌症免疫疗法作为治疗难治性肿瘤的一种可行解决方案受到了关注。然而，由于患者间肿瘤异质性和免疫抑制性肿瘤微环境（TME），其治疗效果仍存在临床局限性。在本研究中，我们证明了不可逆电穿孔（IRE）与干扰素基因刺激剂（STING）激动剂联合可触发抗癌免疫反应。通过体外二维和三维细胞实验确定了通过免疫原性细胞死亡（ICD）诱导损伤相关分子模式（DAMPs）的最佳电条件。在体内同基因肺癌模型中，IRE与STING激动剂联合显示出显著的肿瘤生长抑制作用。我们认为，IRE与STING激动剂的联合策略在有效的癌症免疫治疗方面具有潜力。
