Scheuchenzuber W J, Eskew M L, Zarkower A
Department of Veterinary Science, Pennsylvania State University, University Park 16802.
Exp Lung Res. 1987;13(2):97-112. doi: 10.3109/01902148709064312.
The numbers of specific antibody-forming cells (AFC) in mediastinal lymph nodes (MLN) and spleen were determined in Balb/cByJ mice following inhalation or intratracheal (IT) injection of acetone-dried E. coli or its lipopolysaccharide (LPS). These responses were compared with those obtained using sheep red blood cells (SRBC), an antigen previously used in studies of pulmonary immunity. Inhalation of aerosolized E. coli for as little as 2 min produced significant numbers of AFC in both MLN and spleen, while equivalent administrations of SRBC produced few AFC at either site. Similarly, IT instillations of E. coli resulted in recovery of AFC from MLN and spleen, while IT SRBC produced AFC in MLN but few splenic AFC. IT installations of radiolabeled SRBC and E. coli were used to examine antigen dissemination, and no differences were found in the amounts of radiolabel recovered from various tissues following instillation of either antigen. Experiments using endotoxin resistant mice, and using administrations of LPS in combination with SRBC, were unable to demonstrate alterations in AFC production due to effects of LPS. It was found, however, that inhaled or IT injected E. coli or LPS produced greater pulmonary inflammation than did similar administrations of SRBC, and this may be at least partly responsible for the enhanced induction of systemic immunization.
在Balb/cByJ小鼠吸入或经气管内（IT）注射丙酮干燥的大肠杆菌或其脂多糖（LPS）后，测定纵隔淋巴结（MLN）和脾脏中特异性抗体形成细胞（AFC）的数量。将这些反应与使用绵羊红细胞（SRBC）（一种先前用于肺部免疫研究的抗原）所获得的反应进行比较。吸入雾化大肠杆菌仅2分钟就在MLN和脾脏中产生了大量AFC，而等量的SRBC在这两个部位产生的AFC很少。同样，经气管内注入大肠杆菌可使MLN和脾脏中回收AFC，而经气管内注入SRBC则在MLN中产生AFC，但脾脏中AFC很少。使用放射性标记的SRBC和大肠杆菌进行经气管内注入以检查抗原扩散，在注入任何一种抗原后，从各种组织中回收的放射性标记量未发现差异。使用对内毒素有抗性的小鼠以及将LPS与SRBC联合使用的实验，均未能证明由于LPS的作用而导致AFC产生发生改变。然而，发现吸入或经气管内注射大肠杆菌或LPS比类似的SRBC给药产生更严重的肺部炎症，这可能至少部分是全身免疫诱导增强的原因。
