Belosevic M, Faubert G M, MacLean J D
Parasite Immunol. 1985 Sep;7(5):467-78. doi: 10.1111/j.1365-3024.1985.tb00092.x.
Primary in vitro plaque-forming cell (PFC) responses to sheep red blood cells (SRBC) were examined for spleen and mesenteric lymph node (MLN) cell populations from susceptible (A/J) and resistant (B10.A) mice during the infection with Giardia muris. Spleen and MLN cells isolated from mice during the acute phase of the infection were less responsive to SRBC in vitro than those from uninfected mice. Depressed anti-SRBC PFC response was detected earlier and was more pronounced in MLN cell cultures when compared to the response of spleen cell cultures. Spleen and MLN cells from donors infected with G. muris for 15 days had the capacity of depressing PFC response to SRBC of cells isolated from uninfected mice. This suppressor activity was localized in the plastic-adherent fraction of spleen cell populations isolated from A/J and B10.A mice. Since G. muris is a gastro-intestinal infection of mice, lower capacity of the MLN cells to respond to an antigenic stimulation in vitro may explain, in part, the proliferation of the trophozoites during the acute phase of the infection.
在感染鼠贾第虫期间，检测了易感（A/J）和抗性（B10.A）小鼠的脾脏和肠系膜淋巴结（MLN）细胞群体对绵羊红细胞（SRBC）的体外原发性空斑形成细胞（PFC）反应。在感染急性期从小鼠分离的脾脏和MLN细胞，体外对SRBC的反应比未感染小鼠的细胞弱。与脾细胞培养物的反应相比，抗SRBC PFC反应的抑制在MLN细胞培养物中检测得更早且更明显。感染鼠贾第虫15天的供体的脾脏和MLN细胞，具有抑制从未感染小鼠分离的细胞对SRBC的PFC反应的能力。这种抑制活性定位于从A/J和B10.A小鼠分离的脾细胞群体的塑料贴壁部分。由于鼠贾第虫是小鼠的胃肠道感染，MLN细胞体外对抗抗原刺激的能力较低可能部分解释了感染急性期滋养体的增殖。
