Phung Thuy, Tran Tung, Pham Dung, To Anh, Le Hoa
School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Hanoi, Vietnam.
Ital J Food Saf. 2020 Oct 16;9(3):8772. doi: 10.4081/ijfs.2020.8772.
represents one of the most serious threats to food safety. Several studies have shown that Ready-To- Eat (RTE) meats are an important vehicle responsible for listeriosis in human. In Vietnam, little is known about the occurrence and molecular characteristics of in meat products, which are essential for developing monitoring plans and control measures. In the present study, we investigated the occurrence of in 258 sausage and sliced meat samples collected during the period of 2013-2015 and determined the genetic diversity of the isolates using multi-locus sequence typing (MLST). Overall, was present in 19/129 (14.7 %) and 40/129 (31.0 %) sausage and sliced meat samples respectively, with the peak of occurrence being in summer. Furthermore, a minimum spanning tree was constructed based on MLST data of 47 isolates. A total of 15 sequence types were found, with five being novel. Notably, the majority of the isolates (34/47) belonged to the hypervirulent clonal complexes 1, 2, and 3.
代表了对食品安全最严重的威胁之一。多项研究表明，即食（RTE）肉类是人类李斯特菌病的重要传播媒介。在越南，关于肉类产品中该菌的发生情况和分子特征知之甚少，而这些对于制定监测计划和控制措施至关重要。在本研究中，我们调查了2013年至2015年期间采集的258份香肠和切片肉样本中该菌的发生情况，并使用多位点序列分型（MLST）确定了分离株的遗传多样性。总体而言，该菌分别存在于19/129（14.7%）和40/129（31.0%）的香肠和切片肉样本中，发生高峰在夏季。此外，基于47株分离株的MLST数据构建了最小生成树。共发现15种序列类型，其中5种是新的。值得注意的是，大多数分离株（34/47）属于高毒力克隆复合体1、2和3。
