Kurpas Monika, Osek Jacek, Moura Alexandra, Leclercq Alexandre, Lecuit Marc, Wieczorek Kinga
Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, Pulawy, Poland.
Institut Pasteur, Biology of Infection Unit, Paris, France.
Front Microbiol. 2020 Jun 25;11:1412. doi: 10.3389/fmicb.2020.01412. eCollection 2020.
is one of the major foodborne pathogens. Isolates of PCR-serogroups IIb ( = 17) and IVb ( = 31) recovered from food ( = 33) and food processing environment ( = 15) in Poland were characterized using whole genome sequencing. Most isolates belonged to Multi-Locus Sequence Type (MLST) ST2 (31.3%) and ST5 (22.9%). Core genome MLST (cgMLST) analysis classified isolates into seven sublineages (SL) and 25 different cgMLST types (CT). Consistent with the MLST results, most sublineages were SL2 and SL5. Eleven isolates harbored encoding resistance to aminoglycosides, three isolates harbored ( = 3) and one ( = 1) encoding tolerance to benzalkonium chloride. Isolates belonging to SL5 CT2323 carried a so far unreported allele with a deletion of 141 nucleotides encoding the β-repeat sheet and partially the GW1 domain of InlB. Comparison with publicly available genome sequences from isolated from human listeriosis cases in Poland from 2004 to 2013 revealed five common CTs, suggesting a possible epidemiological link with these strains. The present study contributes to characterize the diversity of in ready-to-eat (RTE) meat and meat processing environments in Poland and unravels previously unnoticed links with clinical cases in Europe.
是主要的食源性病原体之一。使用全基因组测序对从波兰的食品（n = 33）和食品加工环境（n = 15）中分离出的PCR血清群IIb（= 17）和IVb（= 31）菌株进行了特征分析。大多数分离株属于多位点序列类型（MLST）ST2（31.3%）和ST5（22.9%）。核心基因组MLST（cgMLST）分析将分离株分为七个亚系（SL）和25种不同的cgMLST类型（CT）。与MLST结果一致，大多数亚系为SL2和SL5。11株分离株携带编码对氨基糖苷类耐药的基因，3株携带编码对苯扎氯铵耐受的基因（n = 3）和1株（n = 1）。属于SL5 CT2323的分离株携带一个迄今未报道的inlB等位基因，该等位基因缺失了141个核苷酸，编码β重复片层和部分InlB的GW1结构域。与2004年至2013年从波兰人类李斯特菌病病例中分离出的公开可用基因组序列进行比较，发现了五个常见的CT，表明与这些菌株可能存在流行病学联系。本研究有助于描述波兰即食（RTE）肉类和肉类加工环境中李斯特菌的多样性，并揭示与欧洲临床病例以前未被注意到的联系。
