van der Maazen R, de Witte T, Preijers F, Janssen J, Blankenborg G, Wessels H
Department of Radiotherapy, University Hospital, Nijmegen, The Netherlands.
Exp Hematol. 1987 Nov;15(10):1080-5.
The presence of T-lymphocytes in mixed hematopoietic colonies (CFU-MIX) has been reported by some investigators. Though depletion before culturing was performed, residual T cells might be responsible for the observed phenomenon. Using nondepleted marrow or bone marrow depleted to about 2%, T-lymphocytes could be detected in mixed colonies. However, reduction of the T-lymphocytes to less than 0.7% by using a modified E-rosette technique or a cocktail of anti-T-cell monoclonal antibodies (WT1, WT32, WT82) in the presence of baby rabbit complement, resulted in mixed colonies free of T-lymphocytes. After addition of 1.75% T-lymphocytes to this T-cell-depleted bone marrow, T-lymphocytes could be detected in most mixed colonies, but not after the addition of the same percentage of irradiated T-lymphocytes. The presence of T cells in mixed colonies was determined by an adapted immunofluorescence technique (WT32 plus GAM-FITC). The results indicate that mononuclear cells with T-lymphocyte antigens are not the offspring of mixed hematopoietic colony-forming progenitors, but of a low number of T-lymphocytes contaminating the bone marrow after insufficient T-cell depletion.
一些研究人员报告了混合造血集落（CFU-MIX）中T淋巴细胞的存在。尽管在培养前进行了细胞耗竭，但残留的T细胞可能是观察到的现象的原因。使用未耗竭的骨髓或耗竭至约2%的骨髓，可在混合集落中检测到T淋巴细胞。然而，在幼兔补体存在的情况下，通过使用改良的E玫瑰花结技术或抗T细胞单克隆抗体（WT1、WT32、WT82）混合物将T淋巴细胞减少至低于0.7%，可得到不含T淋巴细胞的混合集落。向这种T细胞耗竭的骨髓中加入1.75%的T淋巴细胞后，大多数混合集落中可检测到T淋巴细胞，但加入相同比例的辐照T淋巴细胞后则检测不到。通过改良的免疫荧光技术（WT32加GAM-FITC）确定混合集落中T细胞的存在。结果表明，带有T淋巴细胞抗原的单核细胞不是混合造血集落形成祖细胞的后代，而是在T细胞耗竭不充分后污染骨髓的少量T淋巴细胞的后代。
