刀额新对虾生长激素释放激素基因的克隆与功能鉴定
Molecular cloning and functional characterization of growth hormone-releasing hormone in Mastacembelus armatus.
机构信息
School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and the Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
出版信息
Fish Physiol Biochem. 2021 Feb;47(1):69-78. doi: 10.1007/s10695-020-00895-5. Epub 2020 Oct 28.
Growth hormone-releasing hormone (GHRH) is a neuropeptide that controls growth hormone (GH) synthesis and release. In this study, the full-length cDNA of Mastacembelus armatus ghrh was obtained by rapid amplification of cDNA ends method. Sequence analysis showed that the cloned sequence is 1090 bp in length, containing an open reading frame (ORF) of 429 bp that encodes a precursor protein of 142 amino acids. Sequence alignment revealed that the 27-amino acid mature peptide of Ghrh in M. armatus is conserved. Real-time PCR showed that ghrh is highly expressed in the brain, with very low or no expression in other tissues. During embryonic and larval development, ghrh expression was low in embryos but increased gradually in the stages of larval development. The biological function of Ghrh peptide was further investigated in vivo. Ghrh injection could significantly upregulate the mRNA expression of growth hormone (gh) and insulin-like growth factor-1/2 (igf-1/2) in M. armatus. Our data indicate that Ghrh is able to activate the GH-IGFs axis in M. armatus.
生长激素释放激素（GHRH）是一种神经肽，可控制生长激素（GH）的合成和释放。在这项研究中，通过快速扩增 cDNA 末端法获得了短尾拟鲿 GHRH 的全长 cDNA。序列分析表明，克隆序列长 1090bp，包含一个 429bp 的开放阅读框（ORF），编码 142 个氨基酸的前体蛋白。序列比对显示，M. armatus 的 Ghrh 27 个氨基酸成熟肽保守。实时 PCR 显示，ghrh 在脑中高度表达，在其他组织中表达水平很低或检测不到。在胚胎和幼虫发育过程中，ghrh 在胚胎期的表达水平较低，但在幼虫发育阶段逐渐增加。进一步在体内研究了 Ghrh 肽的生物学功能。Ghrh 注射可显著上调短尾拟鲿生长激素（gh）和胰岛素样生长因子-1/2（igf-1/2）的 mRNA 表达。我们的数据表明，Ghrh 能够激活 M. armatus 的 GH-IGFs 轴。
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