Ivanov E L, Bulat S A, Korolev V G, Koval'tsova S V
Genetika. 1987 Aug;23(8):1383-9.
Induction of forward adenine-dependent (Ade+----Ade-) mutations by HAP was used to analyse genetically yeast mutants with enhanced induced mutagenesis. Three mutations studied in detail segregated as a single mendelian trait and composed independent complementation groups (HIM1, HIM2, HIM3). the him1-1 mutation was centromere-linked, the him3-1 and him2-1 mutations being not. All three mutations did not show any cross-linkage. Uracil-DNA glycosylase activity was determined in crude cell extract from wild type strain and him mutants; no detectable differences were observed.
利用HAP诱导正向腺嘌呤依赖型（Ade+----Ade-）突变来对具有增强诱导诱变作用的酵母突变体进行遗传分析。详细研究的三个突变作为单一孟德尔性状分离，并组成独立的互补群（HIM1、HIM2、HIM3）。him1-1突变与着丝粒连锁，而him3-1和him2-1突变则不连锁。所有这三个突变均未显示任何交叉连锁。在野生型菌株和him突变体的粗细胞提取物中测定了尿嘧啶-DNA糖基化酶活性；未观察到可检测到的差异。
