Herrmann G, Hübner K
Department of Pathology, University of Frankfurt, FRG.
Hepatogastroenterology. 1987 Aug;34(4):148-51.
Liver biopsies of 97 patients with persistent acute hepatitis, a morphologically distinct form of hepatitis with only slightly elevated transaminase values, were screened immunohistochemically for HBs and HBc, and with in-situ hybridisation for HBV DNA. Besides the 37 in part inconstantly immunohistochemically-positive patients, 47 others showed exclusively cytoplasmatic HBV DNA, localizing at least the major part of the replication of viral DNA to the cytoplasm of liver cells. A diffuse distribution pattern of HBV DNA-positive liver cells was accompanied by stronger morphological changes than focally accentuated positivity. In HBc-positive cases, distribution of HBV DNA corresponded mainly to the cytoplasmic type of HBc.
对97例持续性急性肝炎患者的肝活检组织进行了免疫组化检测，以检测HBs和HBc，并通过原位杂交检测HBV DNA。持续性急性肝炎是一种形态学上独特的肝炎形式，转氨酶值仅略有升高。除了37例免疫组化部分呈阳性的患者外，另外47例仅显示细胞质HBV DNA，这至少将病毒DNA复制的主要部分定位于肝细胞的细胞质中。HBV DNA阳性肝细胞的弥漫性分布模式比局灶性强化阳性伴有更明显的形态学变化。在HBc阳性病例中，HBV DNA的分布主要与HBc的细胞质类型相对应。
