Zhao Y, Liang Y R
Department of Pathology, Hunan Medical University, Changsha.
Chin Med J (Engl). 1990 Jul;103(7):556-60.
Liver biopsies of 50 patients with chronic active hepatitis B were analysed immunohistochemically for HBcAg and HBsAg, and, by in situ hybridization, for hepatitis B virus (HBV) DNA. Double staining for manifesting HBV DNA and viral antigen simultaneously was also performed in some of them. The localization patterns of HBV DNA in hepatocytes could be classified into whole cytoplasmic, submembranous, nucleic and intermembranous types. The last type suggests that HBV DNA might be transmitted directly to the adjacent hepatocytes through the cell membranes. The double staining indicated that the majority of hepatocytes with high levels of HBV replication did not contain HBcAg of HBsAg. Conversely, most liver cells with strongly positive HBAg had low or negligible levels of viral replication. In addition, in a few cases HBV DNA could be found in the cytoplasm of bile ductule epithelia and sinusoidal endothelia.
对50例慢性活动性乙型肝炎患者的肝活检组织进行了免疫组织化学分析，检测HBcAg和HBsAg，并通过原位杂交检测乙型肝炎病毒（HBV）DNA。其中部分样本还进行了HBV DNA和病毒抗原的双重染色。肝细胞中HBV DNA的定位模式可分为全细胞质型、膜下型、核型和膜间型。最后一种类型提示HBV DNA可能通过细胞膜直接传递给相邻的肝细胞。双重染色显示，大多数HBV复制水平高的肝细胞不含HBcAg或HBsAg。相反，大多数HBAg强阳性的肝细胞病毒复制水平低或可忽略不计。此外，在少数病例中，在胆小管上皮细胞和窦状内皮细胞的细胞质中可发现HBV DNA。
