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基于串联质量标签（TMT）定量技术的二氧化硅诱导大鼠肺纤维化的比较蛋白质组学分析。

Comparative proteomic analysis of silica-induced pulmonary fibrosis in rats based on tandem mass tag (TMT) quantitation technology.

机构信息

Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, Shandong, China.

Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, Ji'nan, Shandong, China.

出版信息

PLoS One. 2020 Oct 29;15(10):e0241310. doi: 10.1371/journal.pone.0241310. eCollection 2020.

DOI:10.1371/journal.pone.0241310
PMID:33119648
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7595299/
Abstract

Silicosis is a systemic disease characterized by chronic persistent inflammation and incurable pulmonary fibrosis with the underlying molecular mechanisms to be fully elucidated. In this study, we employed tandem mass tag (TMT) based on quantitative proteomics technology to detect differentially expressed proteins (DEPs) in lung tissues of silica-exposed rats. A total of 285 DEPs (145 upregulated and 140 downregulated) were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to predict the biological pathway and functional classification of the proteins. Results showed that these DEPs were mainly enriched in the phagosome, lysosome function, complement and the coagulation cascade, glutathione metabolism, focal adhesion and ECM-receptor interactions. To validate the proteomics data, we selected and analyzed the expression trends of six proteins including CD14, PSAP, GM2A, COL1A1, ITGA8 and CLDN5 using parallel reaction monitoring (PRM). The consistent result between PRM and TMT indicated the reliability of our proteomic data. These findings will help to reveal the pathogenesis of silicosis and provide potential therapeutic targets. Data are available via ProteomeXchange with identifier PXD020625.

摘要

矽肺是一种全身性疾病，其特征为慢性持续性炎症和不可治愈的肺纤维化，但其潜在的分子机制尚未完全阐明。在这项研究中，我们采用基于串联质量标签（TMT）的定量蛋白质组学技术，检测了暴露于二氧化硅的大鼠肺组织中的差异表达蛋白（DEPs）。共鉴定出 285 个 DEPs（145 个上调和 140 个下调）。进行了基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析，以预测蛋白质的生物学途径和功能分类。结果表明，这些 DEPs 主要富集在吞噬体、溶酶体功能、补体和凝血级联、谷胱甘肽代谢、黏附和细胞外基质-受体相互作用。为了验证蛋白质组学数据，我们选择并分析了六个蛋白质的表达趋势，包括 CD14、PSAP、GM2A、COL1A1、ITGA8 和 CLDN5，使用平行反应监测（PRM）。PRM 和 TMT 之间的一致结果表明了我们蛋白质组学数据的可靠性。这些发现将有助于揭示矽肺的发病机制，并为潜在的治疗靶点提供依据。数据可通过 ProteomeXchange 以标识符 PXD020625 获得。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/32465dcda614/pone.0241310.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/33ded6ab7145/pone.0241310.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/31c7190ca805/pone.0241310.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/d44e0501a996/pone.0241310.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/2803e62083f3/pone.0241310.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/747cf614d3d1/pone.0241310.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/32465dcda614/pone.0241310.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/33ded6ab7145/pone.0241310.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/31c7190ca805/pone.0241310.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/d44e0501a996/pone.0241310.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/2803e62083f3/pone.0241310.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/747cf614d3d1/pone.0241310.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dce/7595299/32465dcda614/pone.0241310.g006.jpg

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