Department of Neurobiology, School of Basic Medical Sciences, Army Medical University, Chongqing, China.
Department of Physiology, Southern Medical University, Guangzhou, Guangdong, China.
Methods Mol Biol. 2021;2188:259-271. doi: 10.1007/978-1-0716-0818-0_13.
Intact and functioning brain enables quantification of neural activities directly associated with real world such as visual and auditory information. In vivo patch clamp can record different types of neuronal activity, such as spiking responses, membrane potential dynamics, and synaptic currents (e.g., EPSC, IPSC) in either anesthetized or awake or even free moving animals. Researchers can not only directly measure these neuronal activities but also quantify and unravel synaptic contribution from excitatory and inhibitory circuits. Here, we describe the requirements and standard protocols to perform in vivo patch clamp recording. The key factors of successful recording based on references and our experiences are also provided.
完整而正常运作的大脑能够直接量化与现实世界相关的神经活动，如视觉和听觉信息。在体膜片钳技术可以记录不同类型的神经元活动，如在麻醉或清醒甚至自由活动的动物中记录到的尖峰反应、膜电位动力学和突触电流（如 EPSC、IPSC）。研究人员不仅可以直接测量这些神经元活动，还可以量化和揭示来自兴奋性和抑制性回路的突触贡献。在这里，我们描述了进行在体膜片钳记录的要求和标准方案。根据参考文献和我们的经验，提供了成功记录的关键因素。
