Faculty of Medicine, Department of Physiology, University of Debrecen, Debrecen, Hungary.
Methods Mol Biol. 2025;2857:147-158. doi: 10.1007/978-1-0716-4128-6_14.
Preparation of brain slices for electrophysiological and imaging experiments has been developed several decades ago, and the method is still widely used due to its simplicity and advantages over other techniques. It can be easily combined with other well established and recently developed methods as immunohistochemistry and morphological analysis or opto- and chemogenetics. Several aspects of this technique are covered by a plethora of excellent and detailed review papers, in which one can gain a deep insight of variations in it. In this chapter, I briefly describe the solutions, equipment, and preparation techniques routinely used in our laboratory. I also aim to present how certain "old school" brain slice lab devices can be made in a cost-efficient way. These devices can be easily adapted for the special needs of the experiments. I also aim to present some differences in the preparatory techniques of acutely isolated human brain tissue.
脑片制备技术用于电生理和成像实验已有几十年的历史，由于其简单性以及相对于其他技术的优势，该方法仍然被广泛应用。它可以很容易地与其他成熟和最近开发的方法结合使用，如免疫组织化学和形态分析或光遗传学和化学遗传学。该技术的几个方面都被大量优秀和详细的综述论文所涵盖，人们可以从中深入了解其变化。在本章中，我简要描述了我们实验室常规使用的溶液、设备和制备技术。我还旨在展示如何以具有成本效益的方式制造某些“传统”脑片实验室设备。这些设备可以很容易地适应实验的特殊需求。我还旨在介绍急性分离的人脑组织的制备技术中的一些差异。
