Belford D A, Gole G A, Rush R A
Department of Ophthalmology, Flinders University of South Australia, Bedford Park.
Invest Ophthalmol Vis Sci. 1987 Nov;28(11):1761-6.
Using a whole mount procedure in adult and neonatal mice and adult rats, we have developed an immunohistochemical method for the localization of laminin-like immunoreactivity (LLIR) to the retinal vessels. LLIR was localized to the vascular basement membrane, permitting a clear three-dimensional view of the retinal vasculature. Positive stain was seen in the inner limiting membrane, in retracted capillaries, encasing pericytes, and in a banding pattern on retinal arterioles. The major findings with the whole mount preparations were confirmed using paraffin-embedded material, with the additional observation of LLIR in the lens capsule. In whole mounts of retinas from neonatal mice, LLIR was present from the earliest stages of capillary growth, indicating that laminin is likely to be secreted by endothelial cells during retinal angiogenesis. LLIR within the retinal nerve fiber layer does not precede capillary ingrowth, so no evidence was found that laminin acts as a tracker signal for retinal angiogenesis.
通过在成年和新生小鼠以及成年大鼠中使用整装标本制备方法，我们开发了一种免疫组织化学方法，用于将层粘连蛋白样免疫反应性（LLIR）定位到视网膜血管。LLIR定位于血管基底膜，从而能够清晰地三维观察视网膜血管系统。在内界膜、退缩的毛细血管、包绕的周细胞以及视网膜小动脉的带状模式中可见阳性染色。使用石蜡包埋材料证实了整装标本的主要发现，另外还观察到晶状体囊中有LLIR。在新生小鼠视网膜的整装标本中，从毛细血管生长的最早阶段就存在LLIR，这表明层粘连蛋白可能在视网膜血管生成过程中由内皮细胞分泌。视网膜神经纤维层内的LLIR并不先于毛细血管向内生长，因此没有发现层粘连蛋白作为视网膜血管生成追踪信号的证据。
