Chandrashekar Sangeeta, Vijayakumar Raman, Chelliah Ramachandran, Oh Deog-Hwan
Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, Korea.
Department of Physiology, Bharath Institute of Higher Education and Research, Chennai 600 073, India.
Plants (Basel). 2020 Oct 27;9(11):1445. doi: 10.3390/plants9111445.
The aim of the study was to investigate the antibacterial and anticoagulant activity of Moringa () seed extracts and coagulant protein for their potential application in water treatment. Pathogenic microorganisms were obtained from Ramachandra Hospital, Chennai, India. Bacterial cell aggregation and growth kinetics studies were employed for six bacterial strains with different concentrations of seed extracts and coagulant protein. Moringa seed extract and coagulant protein showed cell aggregation against six bacterial strains, whereas seed extract alone showed growth inhibition of all six bacterial strains for up to 6 h, compared to that of control. and B did not develop resistance against coagulant protein. The results imply that is likely an efficient low-molecular bioactive peptide (with <7.5 kDa plant-based coagulant and antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography-mass spectrometry and HPLC, with the corresponding sequences from Napin-1A peptide posing different degrees of antibacterial activity against different pathogenic organisms.
本研究的目的是调查辣木种子提取物和凝血蛋白的抗菌及抗凝活性，以探讨其在水处理中的潜在应用。致病微生物取自印度钦奈的罗摩衍那医院。采用细菌细胞聚集和生长动力学研究方法，对六种细菌菌株使用不同浓度的种子提取物和凝血蛋白进行研究。辣木种子提取物和凝血蛋白对六种细菌菌株均表现出细胞聚集作用，而单独的种子提取物在长达6小时内对所有六种细菌菌株均表现出生长抑制作用，与对照组相比。A和B对凝血蛋白未产生抗性。结果表明，通过液相色谱-质谱联用和高效液相色谱法证实，辣木可能是一种高效的低分子生物活性肽（具有小于7.5 kDa的植物基凝血剂和抗菌肽），其来自Napin-1A肽的相应序列对不同致病生物具有不同程度的抗菌活性。
