Nafeh Amany Abd El-Shafy Abd El-Kader, Mohamed Ibrahim Mohamed Abd El-Aleem, Foda Mohamed Frahat
Department of Biochemistry, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, Egypt.
National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Nanomaterials (Basel). 2024 Jul 26;14(15):1254. doi: 10.3390/nano14151254.
() is globally recognized for its medicinal properties and offers high-quality, protein-rich seeds. This study aimed to explore the potential of seeds as a significant source of protein-based nanoparticles (PBNPs) using the ultrasonication technique after desolvation and to evaluate their cytotoxicity in the human leukemia cell line (THP-1) for the first time. The properties of the PBNPs were confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR). The extracted protein from moringa seed cake flour had a significant protein content of 54.20%, and the resulting PBNPs had an average size of 134.3 ± 0.47 nm with a robust zeta potential of -43.15 mV. Notably, our study revealed that PBNPs exhibited cytotoxic potential at high concentrations, especially against the THP-1 human leukemia cell line, which is widely used to study immunomodulatory properties. The inhibitory effect of PBNPs was quantitatively evidenced by a cytotoxicity assay, which showed that a concentration of 206.5 μg mL (log conc. 2.315) was required to inhibit 50% of biological activity. In conclusion, our findings highlight the potential of seeds as a valuable resource in the innovative field of eco-friendly PBNPs by combining traditional medicinal applications with contemporary advancements in protein nanotechnology. However, further studies are required to ensure their biocompatibility.
（某植物）因其药用特性而在全球范围内得到认可，并提供高质量、富含蛋白质的种子。本研究旨在探索在去溶剂化后使用超声技术将（该植物）种子作为基于蛋白质的纳米颗粒（PBNPs）的重要来源的潜力，并首次评估其对人白血病细胞系（THP-1）的细胞毒性。通过动态光散射（DLS）、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDX）、X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）对PBNPs的特性进行了确认。从辣木籽饼粉中提取的蛋白质的蛋白质含量显著为54.20%，所得PBNPs的平均尺寸为134.3±0.47 nm，具有-43.15 mV的强zeta电位。值得注意的是，我们的研究表明，PBNPs在高浓度下表现出细胞毒性潜力，尤其是对广泛用于研究免疫调节特性的THP-1人白血病细胞系。细胞毒性试验定量证明了PBNPs的抑制作用，该试验表明，需要206.5μg/mL（对数浓度2.315）的浓度来抑制50%的生物活性。总之，我们的研究结果突出了（该植物）种子通过将传统药用应用与蛋白质纳米技术的当代进展相结合，在生态友好型PBNPs创新领域作为宝贵资源的潜力。然而，需要进一步研究以确保其生物相容性。
