ICAR-Indian Institute of Rice Research, Hyderabad 500030, India.
Bioinformatics Lab, ICAR-National Academy of Agricultural Research Management, Hyderabad 500030, India.
Int J Mol Sci. 2020 Oct 27;21(21):7974. doi: 10.3390/ijms21217974.
MicroRNAs regulate plant responses to fungal infections and immunity. In this study, miRNAs were identified in six rice cultivars during a Kühn AG1-IA infection using a deep sequencing approach. Known and novel miRNAs were analyzed in these rice cultivars, and a set of fungal infection/immunity-associated miRNAs and target genes were quantified by reverse transcription (RT)-qPCR in six rice cultivars. Additionally, the relative expression of these miRNAs was analyzed in different time points of the infection, wild species of rice, and in response to different strains of . Osa-miR1320-5p showed preferential expression during the fungal infection in all the six rice genotypes, while Osa-miR156d, Osa-miR159b, Osa-miR820c, and Osa-miR1876 were differentially regulated in susceptible and resistant genotypes. A greater degree of downregulation of miRNAs was observed during the initial time points of infection (24-72 h), suggesting a maximum molecular activity of rice- interaction and resistance response of the host during the early phase of infection. After infection, the expression of Osa-miR820c and Osa-miR156d was downregulated in and , while Osa-miR397b was downregulated in all the wild rice species except . This study provided comprehensive information on the repertoire of miRNAs expressed in six sheath blight disease-susceptible and resistant and aus rice cultivars.
MicroRNAs 调控植物对真菌侵染和免疫的反应。在这项研究中，我们采用深度测序方法鉴定了 6 个水稻品种在 Kühn AG1-IA 感染过程中的 miRNAs。我们分析了这些水稻品种中的已知和新的 miRNAs，并通过反转录（RT）-qPCR 定量检测了 6 个水稻品种中一组与真菌侵染/免疫相关的 miRNAs 和靶基因。此外，我们还分析了这些 miRNAs 在感染不同时间点、不同野生稻种以及对不同菌株的相对表达情况。Osa-miR1320-5p 在所有 6 个水稻基因型的真菌感染过程中表现出优先表达，而 Osa-miR156d、Osa-miR159b、Osa-miR820c 和 Osa-miR1876 在感病和抗病基因型中差异表达。在感染的初始时间点（24-72 h）观察到 miRNAs 的下调程度更大，这表明在感染的早期阶段，水稻与病原菌相互作用和宿主抗性反应的分子活性达到最大值。在 感染后，Osa-miR820c 和 Osa-miR156d 的表达在 和 中下调，而 Osa-miR397b 在除 之外的所有野生稻种中下调。本研究提供了在 6 个叶鞘枯病易感和抗性 和 aus 水稻品种中表达的 miRNAs 谱的全面信息。
