Department of Bio and Nano Technology, Guru Jambheshwar University of Science and Technology, Hisar, INDIA.
Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite, Ethiopia.
RNA Biol. 2022;19(1):519-532. doi: 10.1080/15476286.2022.2062172. Epub 2021 Dec 31.
In the recent past, cross-kingdom movement of miRNAs, small (20-25 bases), and endogenous regulatory RNA molecules has emerged as one of the major research areas to understand the potential implications in modulating the plant's biotic stress response. The current review discussed the recent developments in the mechanism of cross-kingdom movement (long and short distance) and critical cross-talk between host's miRNAs in regulating gene function in bacteria, fungi, viruses, insects, and nematodes, and during host-pathogen interaction and their potential implications in crop protection. Moreover, cross-kingdom movement during symbiotic interaction, the emerging role of plant's miRNAs in modulating animal's gene function, and feasibility of spray-induced gene silencing (SIGS) in combating biotic stresses in plants are also critically evaluated. The current review article analysed the horizontal transfer of miRNAs among plants, animals, and microbes that regulates gene expression in the host or pathogenic organisms, contributing to crop protection. Further, it highlighted the challenges and opportunities to harness the full potential of this emerging approach to mitigate biotic stress efficiently.
在最近的一段时间里，miRNAs（小的（20-25 个碱基）和内源性调节 RNA 分子）的跨界转移已成为一个主要的研究领域，以了解其在调节植物生物胁迫反应方面的潜在意义。本综述讨论了跨界转移（长距离和短距离）的最新发展，以及宿主 miRNA 之间的关键串扰，以调节细菌、真菌、病毒、昆虫和线虫中的基因功能，以及在宿主-病原体相互作用及其在作物保护中的潜在意义。此外，还对共生相互作用过程中的跨界转移、植物 miRNA 在调节动物基因功能中的新兴作用，以及喷雾诱导基因沉默（SIGS）在防治植物生物胁迫方面的可行性进行了批判性评估。本文分析了 miRNA 在植物、动物和微生物之间的水平转移，这些转移调节宿主或病原生物中的基因表达，有助于作物保护。此外，还强调了利用这一新兴方法的潜力来有效减轻生物胁迫的挑战和机遇。
