黄芪多糖通过调节骨髓间充质干细胞（BMSCs）凋亡抑制辐射诱导的旁观者效应。
Astragalus polysaccharide inhibits radiation-induced bystander effects by regulating apoptosis in Bone Mesenchymal Stem Cells (BMSCs).
机构信息
Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities, Gansu University of Chinese Medicine , Lanzhou, China.
Key Laboratory of Space Radiobiology of Gansu Province & Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, Chinese Academy of Sciences , Lanzhou, China.
出版信息
Cell Cycle. 2020 Nov;19(22):3195-3207. doi: 10.1080/15384101.2020.1838793. Epub 2020 Oct 30.
Abstract
The purpose of this study was to investigate the effects of astragalus polysaccharides (APS) on the proliferation and apoptosis of bone marrow mesenchymal stem cells (BMSCs) induced by X-ray radiation-induced A549 cells bystander effect (RIBE), and to explore their mechanisms. In this study, APS increased the reduced cell proliferation rate induced by RIBE and inhibiting the apoptosis of bystander cells. In terms of mechanism, APS up-regulates the proteins Bcl-2, Bcl-xl, and down-regulates the proteins Bax and Bak, which induces a decrease in mitochondrial membrane potential, which induces the release of Cyt-c and AIF, which leads to caspase-dependent and caspase-independent pathway to cause apoptosis. In addition, we believe that ROS may be the main cause of these protein changes. APS can inhibit the generation of ROS in bystander cells and thus inhibit the activation of the mitochondrial pathway, further preventing cellular damage caused by RIBE.
摘要
本研究旨在探讨黄芪多糖（APS）对 X 射线辐射诱导的 A549 细胞旁效应（RIBE）诱导的骨髓间充质干细胞（BMSCs）增殖和凋亡的影响，并探讨其机制。本研究发现，APS 可增加 RIBE 诱导的细胞增殖率降低，并抑制旁观者细胞凋亡。在机制方面，APS 上调 Bcl-2、Bcl-xl 蛋白，下调 Bax 和 Bak 蛋白，导致线粒体膜电位降低，引起 Cyt-c 和 AIF 的释放，从而通过 caspase 依赖和非依赖途径引起细胞凋亡。此外，我们认为 ROS 可能是这些蛋白变化的主要原因。APS 可以抑制旁观者细胞中 ROS 的产生，从而抑制线粒体途径的激活，进一步防止 RIBE 引起的细胞损伤。
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