Uhteg Katharine, Carroll Karen C, Mostafa Heba H
Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Meyer B-121F, 600 North Wolfe Street, Baltimore, MD 21287-7093, USA.
Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Meyer B-121F, 600 North Wolfe Street, Baltimore, MD 21287-7093, USA.
Clin Lab Med. 2020 Dec;40(4):459-472. doi: 10.1016/j.cll.2020.08.004. Epub 2020 Aug 8.
Endemic species of coronavirus (HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoV-HKU1) are frequent causes of upper respiratory tract infections. Three highly pathogenic coronaviruses have been associated with outbreaks and epidemics and have challenged clinical microbiology laboratories to quickly develop assays for diagnosis. Their initial characterization was achieved by molecular methods. With the great advance in metagenomic whole-genome sequencing directly from clinical specimens, diagnosis of novel coronaviruses could be quickly implemented into the workflow of managing cases of pneumonia of unknown cause, which will markedly affect the time of the initial characterization and accelerate the initiation of outbreak control measures.
冠状病毒的地方性物种（人冠状病毒OC43、人冠状病毒229E、人冠状病毒NL63和人冠状病毒HKU1）是上呼吸道感染的常见病因。三种高致病性冠状病毒与疫情爆发有关，对临床微生物实验室快速开发诊断检测方法构成了挑战。它们最初的特征是通过分子方法确定的。随着直接从临床标本进行宏基因组全基因组测序的巨大进展，新型冠状病毒的诊断可以迅速纳入不明原因肺炎病例管理的工作流程中，这将显著影响最初特征描述的时间，并加速疫情控制措施的启动。
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