College of Animal Science and Technology, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
Department of Pediatric Neurology, The First Hospital of Jilin University, Changchun, Jilin Province, 130021, PR China.
Microb Pathog. 2020 Dec;149:104577. doi: 10.1016/j.micpath.2020.104577. Epub 2020 Oct 27.
Aeromous veronii is a severe pathogen that can infect aquatic organisms and mammals also causes irreparable damage to fish aquaculture. Analysis of the results of epidemiological investigations have revealed that its tolerance to drugs and the virulence of A. veronii have increased in recent years. Most of the researches on A. veronii focuse on the strain isolation, identification, and drug susceptibility. However, we do not know so much about the molecular mechanism of the pathogenesis on A. veronii. Here we identified and obtained the highly expressed TH0426 Nucleoside Diphosphate Kinases (NDK) of A. veronii. We first constructed a mutant strain (△-ndk) by generating an in-frame deletion of the ndk gene, to investigate the functional role in A. veronii TH0426. The ability in the adhesion and invasion of EPC cells and biofilm formation significantly reduced of the △-ndk strain. The motility test showed that the ndk gene affected on the swimming ability, while did not affect the swarming motility. Compared with the wild-type strain TH0426, the pathogenicity of △-ndk strain to zebrafish reduced severely. Besides, the ndk gene has affected the apoptosis rate of A. veronii TH0426. These results would help to demonstrate the function of ndk further and realize the pathogenesis on A. veronii.
维罗纳气单胞菌是一种严重的病原体，可感染水生生物和哺乳动物，也会对鱼类养殖业造成不可挽回的损害。对流行病学调查结果的分析表明，近年来，该菌对药物的耐受性和毒力有所增加。大多数关于维罗纳气单胞菌的研究都集中在菌株的分离、鉴定和药敏上。然而，我们对维罗纳气单胞菌的发病机制知之甚少。在这里，我们鉴定并获得了维罗纳气单胞菌 TH0426 的高度表达的核苷二磷酸激酶（NDK）。我们首先通过产生 ndk 基因的框内缺失来构建突变株（△-ndk），以研究其在 A. veronii TH0426 中的功能作用。该△-ndk 菌株对 EPC 细胞的黏附和侵袭能力以及生物膜形成能力显著降低。运动性测试表明，ndk 基因影响游泳能力，但不影响群集运动能力。与野生型 TH0426 菌株相比，△-ndk 菌株对斑马鱼的致病性大大降低。此外，ndk 基因影响了 A. veronii TH0426 的细胞凋亡率。这些结果有助于进一步证明 ndk 的功能，并实现对维罗纳气单胞菌发病机制的认识。
