Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
J Fish Dis. 2022 Feb;45(2):231-247. doi: 10.1111/jfd.13544. Epub 2021 Dec 7.
Aeromonas veronii (A. veronii) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Based on our previous studies on proteomics and genomics, we found out that the aodp gene may be related to the virulence of A. veronii TH0426. However, aodp gene encodes a hypothetical protein with an unknown function, and its role in A. veronii TH0426 is not clear. Here, we first constructed a mutant strain (△-aodp) to investigate the functional role of aodp in A. veronii TH0426. Compared with the wild strain A. veronii TH0426, the growth rate of strain △-aodp was slower and was resistant to neomycin and kanamycin, but sensitive to cephalexin. The swimming and swarming ability of △-aodp strain decreased, and the pathogenicity to mice decreased by 15.84-fold. Besides, the activity of caspase-3 in EPCs infected with △-aodp strain was 1.49-fold lower than that of the wild strain. We examined 20 factors closely related to A. veronii virulence, among them 17 genes were down-regulated as a result of aodp deficiency. This study laid a foundation for further studies on the pathogenesis of A. veronii.
维氏气单胞菌（Aeromonas veronii，A. veronii）是一种鱼类、人类和牲畜的机会致病菌，对水产养殖业的发展构成威胁。基于我们之前在蛋白质组学和基因组学方面的研究，我们发现 aodp 基因可能与 A. veronii TH0426 的毒力有关。然而，aodp 基因编码一种具有未知功能的假设蛋白，其在 A. veronii TH0426 中的作用尚不清楚。在这里，我们首先构建了一个突变株（△-aodp），以研究 aodp 在 A. veronii TH0426 中的功能作用。与野生型 A. veronii TH0426 相比，突变株△-aodp 的生长速度较慢，对新霉素和卡那霉素有抗性，但对头孢氨苄敏感。△-aodp 菌株的泳动和群集能力下降，对小鼠的致病性降低了 15.84 倍。此外，感染△-aodp 菌株的 EPCs 中 caspase-3 的活性比野生型菌株低 1.49 倍。我们检测了与 A. veronii 毒力密切相关的 20 个因素，其中 17 个基因因 aodp 缺失而下调。这项研究为进一步研究 A. veronii 的发病机制奠定了基础。
