酵母中tRNA连接酶的亚核定位。
The subnuclear localization of tRNA ligase in yeast.
作者信息
Clark M W, Abelson J
机构信息
Division of Biology, California Institute of Technology, Pasadena 91125.
出版信息
J Cell Biol. 1987 Oct;105(4):1515-26. doi: 10.1083/jcb.105.4.1515.
Abstract
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location to be primarily at the inner membrane of the nuclear envelope, most likely at the nuclear pore. There is a more diffuse, secondary location of ligase in a region of the nucleoplasm within 300 nm of the nuclear envelope. When the amount of ligase in the cell is increased, nuclear staining increases but staining of the nuclear envelope remains constant. This experiment indicates that there are a limited number of ligase sites at the nuclear envelope. Since the other tRNA splicing component, the endonuclease, has the characteristics of an integral membrane protein, we hypothesize that it constitutes the site for the interaction of ligase with the nuclear envelope.
摘要
酵母tRNA连接酶是tRNA剪接所需的一种酶。一项间接免疫荧光研究表明，这种酶定位于细胞核中。在更高分辨率下，使用间接免疫电子显微镜的研究表明，这种核定位主要在核膜的内膜，最有可能在核孔处。连接酶在核膜内300纳米范围内的核质区域有一个更弥散的次要定位。当细胞中连接酶的量增加时，核染色增加，但核膜染色保持不变。该实验表明核膜上连接酶位点的数量有限。由于另一种tRNA剪接成分——核酸内切酶具有整合膜蛋白的特征，我们推测它构成了连接酶与核膜相互作用的位点。
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