Oriol R, Mancilla-Jimenez R
J Immunol Methods. 1983 Aug 26;62(2):185-92. doi: 10.1016/0022-1759(83)90245-4.
Mounting immunofluorescent slides in an oxidized solution of p-phenylenediamine in 90% glycerol, resulted in brown fluorescent staining of nuclei. This contrasts well with the fluorescence of fluorescein and rhodamine conjugates and facilitates excellent morphological localization of antigens. The antifading effect of p-phenylenediamine is maintained in this oxidized solution. In addition, amyloid substance gives a bright yellow-orange fluorescence with this medium. This method of staining is more sensitive and less time consuming than the usual methods for amyloid. Alternative techniques to keep the antifading effect without either nuclear or amyloid counterstain (for identification of nuclear antigens), or to show up amyloid deposits without nuclear counterstain (for small amyloid deposits) are described.
将免疫荧光载玻片置于对苯二胺在90%甘油中的氧化溶液中封固，可使细胞核呈棕色荧光染色。这与荧光素和罗丹明缀合物的荧光形成鲜明对比，有助于对抗抗原进行出色的形态学定位。对苯二胺的抗褪色作用在这种氧化溶液中得以保持。此外，淀粉样物质在这种介质中呈现亮黄橙色荧光。这种染色方法比常规的淀粉样物质染色方法更灵敏且耗时更少。本文还描述了在不进行核或淀粉样物质复染（用于鉴定核抗原）的情况下保持抗褪色效果的替代技术，或者在不进行核复染的情况下显示淀粉样沉积物（用于小淀粉样沉积物）的替代技术。
