van de Water Jeroen A J M, Coppari Martina, Enrichetti Francesco, Ferrier-Pagès Christine, Bo Marzia
Centre Scientifique de Monaco, Monaco, Monaco.
Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, Università degli Studi di Genova, Genova, Italy.
Front Microbiol. 2020 Oct 6;11:537813. doi: 10.3389/fmicb.2020.537813. eCollection 2020.
Black corals are important habitat-forming species in the mesophotic and deep-sea zones of the world's oceans because of their arborescent colony structure and tendency to form animal forests. Although we have started unraveling the ecology of mesophotic black corals, the importance of the associated microbes to their health has remained unexplored. Here, we provide in-depth assessments of black coral-microbe symbioses by investigating the spatial and temporal stability of these associations, and make comparisons with a sympatric octocoral with similar colony structure. To this end, we collected samples of colonies from three mesophotic shoals situated along the Ligurian Coast of the Mediterranean Sea (Bordighera, Portofino, Savona) in the spring of 2017. At the Portofino shoal, samples of and the gorgonian were collected in November 2016 and May 2017. Bacterial communities were profiled using 16S rRNA gene amplicon sequencing. The bacterial community of was consistently dominated by . Contrastingly, the black coral microbiome was more diverse, and was primarily composed of numerous Bacteroidetes, Alpha- and Gammaproteobacterial taxa, putatively involved in all steps of the nitrogen and sulfur cycles. Compositional differences in the microbiome existed between all locations and both time points, and no phylotypes were consistently associated with . This highlights that local conditions may influence the bacterial community structure and potentially nutrient cycling within the holobiont. But it also suggests that this coral holobiont possesses a high degree of microbiome flexibility, which may be a mechanism to acclimate to environmental change.
由于其树状群体结构以及形成动物森林的倾向，黑珊瑚是世界海洋中光中带和深海区域重要的栖息地形成物种。尽管我们已经开始了解光中带黑珊瑚的生态学，但与之相关的微生物对其健康的重要性仍未得到探索。在这里，我们通过研究这些共生关系的空间和时间稳定性，对黑珊瑚与微生物的共生关系进行了深入评估，并与具有相似群体结构的同域八放珊瑚进行了比较。为此，我们于2017年春季从地中海利古里亚海岸沿线的三个光中带浅滩（博尔迪盖拉、波托菲诺、萨沃纳）采集了群体样本。在波托菲诺浅滩，2016年11月和2017年5月采集了 和柳珊瑚的样本。使用16S rRNA基因扩增子测序对细菌群落进行了分析。 的细菌群落一直以 为主。相比之下，黑珊瑚的微生物群落更加多样化，主要由众多拟参与氮和硫循环所有步骤的拟杆菌门、α-和γ-变形菌纲分类群组成。 的微生物群落在所有地点和两个时间点之间都存在组成差异，并且没有系统型与 始终相关。这突出表明当地条件可能会影响 全生物体内的细菌群落结构以及潜在的营养循环。但这也表明这种珊瑚全生物体具有高度的微生物群落灵活性，这可能是一种适应环境变化的机制。
