State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Rd., Hangzhou City 310003, China.
National Clinical Research Center for Infectious Diseases, 79 Qingchun Rd., Hangzhou City 310003, China.
Biomed Res Int. 2020 Oct 14;2020:1673602. doi: 10.1155/2020/1673602. eCollection 2020.
Liver injury has caused significant illness in humans worldwide. The dynamics of intestinal bacterial communities associated with natural recovery and therapy for CCl-treated liver injury remain poorly understood. This study was designed to determine the recovery dynamics of intestinal bacterial communities in CCl-treated mice with or without mesenchymal stem cell transplantation (i.e., MSC and CCl groups) at 48 h, 1 week (w), and 2 w. MSCs significantly improved the histopathology, survival rate, and intestinal structural integrity in the treated mice. The gut bacterial communities were determined with significant changes in both the MSC and CCl groups over time, with the greatest difference between the MSC and CCl groups at 48 h. The liver injury dysbiosis ratio experienced a decrease in the MSC groups and a rise in the CCl groups over time, suggesting the mice in the MSC group at 48 h and the CCl group at two weeks were at the least gut microbial dysbiosis status among the corresponding cohorts. Multiple OTUs and functional categories were associated with each of the bacterial communities in the MSC and CCl groups over time. Among these gut phylotypes, OTU1352_ was determined as the vital member in MSC-treated mice at 48 h, while OTU453_, OTU1213_Ruminococcaceae, and OTU841_ were determined as the vital phylotypes in CCl-treated mice at two weeks. The relevant findings could assist the diagnosis of the microbial dysbiosis status of intestinal bacterial communities in the CCl-treated cohorts with or without MSC transplantation.
肝损伤已在全球范围内导致大量人类患病。与 CCl 处理的肝损伤的自然恢复和治疗相关的肠道细菌群落的动态仍知之甚少。本研究旨在确定在 MSC 移植（即 MSC 和 CCl 组）存在或不存在的情况下，CCl 处理的小鼠的肠道细菌群落的恢复动态，时间点为 48 小时、1 周和 2 周。MSC 显著改善了治疗小鼠的组织病理学、存活率和肠道结构完整性。肠道细菌群落随时间在 MSC 和 CCl 组中均发生了显著变化，MSC 和 CCl 组之间在 48 小时时差异最大。随着时间的推移，肝损伤失调比在 MSC 组中下降，在 CCl 组中上升，这表明 MSC 组在 48 小时时和 CCl 组在两周时处于相应队列中肠道微生物失调状态最低。多个 OTUs 和功能类别与 MSC 和 CCl 组中每个细菌群落随时间的变化相关。在这些肠道类群中，OTU1352_被确定为 MSC 治疗的小鼠在 48 小时时的重要成员，而 OTU453_、OTU1213_Ruminococcaceae 和 OTU841_被确定为 CCl 治疗的小鼠在两周时的重要类群。这些相关发现可以帮助诊断 CCl 处理的有或没有 MSC 移植的队列中肠道细菌群落的微生物失调状态。
