Department of Molecular Biology and Genetics, Science Faculty, Sivas Cumhuriyet University, Sivas, Turkey.
Curr Microbiol. 2021 Jan;78(1):144-149. doi: 10.1007/s00284-020-02261-3. Epub 2020 Oct 29.
One-dodecanol was identified to be the predominant secondary metabolite of a novel isolate (S15) of Streptomyces griseobrunneus. For its demonstration, secondary metabolite extracts were electrophoresed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). A yellowish unique band was then cut out from the gel and its metabolite content was eluted in n-butanol. GC-MS analysis indicated that more than 93% of the of the elution material were 1-dodecanol. The compound was further characterized by FTIR and C NMR analyses. Dendrogram built on the basis of 16S rRNA gene sequence indicated that the isolate S15 was a member of Streptomyces griseobrunneus.
十二烷醇被鉴定为新型灰色链霉菌（S15）的主要次生代谢产物。为了证明这一点，对次生代谢产物提取物进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）电泳。然后从凝胶中切下一条黄色独特的条带，并在正丁醇中洗脱其代谢产物。GC-MS 分析表明，洗脱材料中超过 93%的是 1-十二烷醇。该化合物进一步通过傅里叶变换红外光谱（FTIR）和碳核磁共振（C NMR）分析进行了表征。基于 16S rRNA 基因序列构建的系统发育树表明，分离株 S15 是灰色链霉菌的一个成员。
