Department of Orthopedics, Bayannaoer City Hospital, Bayannaoer, Inner Mongolia 015000, P.R. China.
Department of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, P.R. China.
Int J Mol Med. 2020 Dec;46(6):2260-2270. doi: 10.3892/ijmm.2020.4739. Epub 2020 Sep 25.
The aim of the present study was to assess the activation of nuclear factor‑κB (NF‑κB) in the infrapatellar fat pads (IPFPs) of obese patients with knee osteoarthritis (KOA). For this purpose, 32 patients (22 obese patients with KOA and 10 patients with KOA with a healthy weight) treated with total knee arthroplasty (TKA) were selected. The expression levels of pro‑inflammatory cytokines and adipocytokines, and the activation of NF‑κB were detected in both the cases and controls by enzyme‑linked immunosorbent assay (ELISA), western blot analysis and immunohistochemistry where appropriate. SPSS 18.0 software was used for statistical analysis to determine the correlation between obesity and the detected cytokine levels. It was found that in patients with KOA, the expression of leptin in the synovial fluid positively correlated with body mass index (BMI; P<0.05), and the expression of interleukin (IL)‑6 in serum significantly correlated with the IL‑1β, leptin and tumor necrosis factor (TNF)‑α levels (P<0.05). Furthermore, the expression of inflammatory cytokines and adipocytokines in IPFPs differed significantly between the obese and non‑obese patients with KOA (P<0.05). By evaluating the expression of IKKβ and IκBα and the nuclear translocation ability of p‑p65, it was concluded that NF‑κB signaling was activated to a higher degree in the IPFP tissues of obese patients with KOA than in those of patients with KOA with a healthy weight. On the whole, the findings of the present study suggested that the NF‑κB signaling pathway was activated and that there were changes in the expression in levels of inflammatory cytokines and adipocytokines in the IPFP tissues of obese patients with KOA.
本研究旨在评估肥胖膝骨关节炎（KOA）患者髌下脂肪垫（IPFP）中核因子-κB（NF-κB）的激活情况。为此，选择了 32 名接受全膝关节置换术（TKA）治疗的患者（22 名肥胖 KOA 患者和 10 名体重健康的 KOA 患者）。通过酶联免疫吸附试验（ELISA）、western blot 分析和免疫组织化学法适当检测病例和对照中促炎细胞因子和脂肪细胞因子的表达水平以及 NF-κB 的激活情况。使用 SPSS 18.0 软件进行统计分析，以确定肥胖与检测到的细胞因子水平之间的相关性。结果发现，在 KOA 患者中，滑液中瘦素的表达与体重指数（BMI）呈正相关（P<0.05），血清中白细胞介素（IL）-6 的表达与 IL-1β、瘦素和肿瘤坏死因子（TNF）-α水平显著相关（P<0.05）。此外，肥胖和非肥胖 KOA 患者的 IPFPs 中炎症细胞因子和脂肪细胞因子的表达存在显著差异（P<0.05）。通过评估 IKKβ和 IκBα的表达以及 p-p65 的核转位能力，得出结论，肥胖 KOA 患者的 IPFP 组织中 NF-κB 信号通路的激活程度高于体重健康的 KOA 患者。总的来说，本研究的结果表明，NF-κB 信号通路被激活，并且在肥胖 KOA 患者的 IPFP 组织中炎症细胞因子和脂肪细胞因子的表达水平发生了变化。
